Introduction of the gene amplification technique to decrease the risk of hepatitis C virus transmission by plasma products

被引：4

作者：

Branovic, K ^{[1
]}

Forcis, D ^{[1
]}

Kosutic-Gulija, T ^{[1
]}

Santak, M ^{[1
]}

Zgorelec, R ^{[1
]}

Mazuran, R ^{[1
]}

机构：

[1] Univ Zagreb, Inst Immunol, Zagreb, Croatia

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 852卷 / 01期

关键词：

hepatitis C virus; viruses; RNA;

D O I：

10.1016/S0021-9673(99)00468-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The viral safety of plasma-derived products with respect to hepatitis C virus (HCV) is assured by selection of donors, screening of individual donations for antibodies to HCV and the incorporation of effective viral inactivation-removal steps into manufacturing processes. As antibody screening of single donations is not sufficient to completely eliminate HCV RNA positive plasmas from plasma pools, testing for HCV RNA by gene amplification techniques may be necessary to identify positive donations. Using modem molecular biology techniques, we developed a specific, sensitive and reproducible method for routine PCR screening for HCV RNA in plasma pools. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：305 / 312

页数：8

共 10 条

[1] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[2] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME
CHOO, QL
KUO, G
WEINER, AJ
OVERBY, LR
BRADLEY, DW
HOUGHTON, M
[J]. SCIENCE, 1989, 244 (4902) : 359 - 362
[3] International collaborative study on the second EUROHEP HCV-RNA reference panel
Damen, M
Cuypers, HTM
Zaaijer, HL
Reesink, HW
Schaasberg, WP
Gerlich, WH
Niesters, HGM
Lelie, PN
[J]. JOURNAL OF VIROLOGICAL METHODS, 1996, 58 (1-2) : 175 - 185
[4] GARSON JA, 1994, FEMS MICROBIOL REV, V14, P229, DOI 10.1111/j.1574-6976.1994.tb00093.x
[5] A sensitive PCR assay system for the quantitation of viral genome equivalents: Hepatitis C virus (HCV)
Hammerle, T
Falkner, FG
Dorner, F
[J]. ARCHIVES OF VIROLOGY, 1996, 141 (11) : 2103 - 2114
[6] AVOIDING FALSE POSITIVES WITH PCR
KWOK, S
HIGUCHI, R
[J]. NATURE, 1989, 339 (6221) : 237 - 238
[7] An internal control for routine diagnostic PCR: Design, properties, and effect on clinical performance
Rosenstraus, M
Wang, Z
Chang, SY
DeBonville, D
Spadoro, JP
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) : 191 - 197
[8] Incidence of hepatitis C virus RNA in anti-HCV-negative plasma pools and blood products
Saldanha, J
Minor, P
[J]. VOX SANGUINIS, 1996, 70 (04) : 232 - 234
[9] Collaborative study to assess the suitability of an HCV RNA reference sample for detection of an HCV RNA in plasma pools by PCR
Saldanha, J
Minor, P
[J]. VOX SANGUINIS, 1996, 70 (03) : 148 - 151
[10] RELIABILITY OF POLYMERASE CHAIN-REACTION FOR DETECTION OF HEPATITIS-C VIRUS
ZAAIJER, HL
CUYPERS, HTM
REESINK, HW
WINKEL, IN
GERKEN, G
LELIE, PN
[J]. LANCET, 1993, 341 (8847) : 722 - 724

← 1 →