Real-time, continuous, fluorescence sensing in a freely-moving subject with an implanted hybrid VCSEL/CMOS biosensor

被引：17

作者：

O'Sullivan, Thomas D. ^{[1
]}

Heitz, Roxana T. ^{[1
]}

Parashurama, Natesh ^{[2
,3
]}

Barkin, David B. ^{[4
]}

Wooley, Bruce A. ^{[1
]}

Gambhir, Sanjiv S. ^{[2
,3
]}

Harris, James S. ^{[1
]}

Levi, Ofer ^{[5
,6
]}

机构：

[1] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA

[2] Stanford Univ, Mol Imaging Program Stanford, Dept Radiol, BioX Program, Stanford, CA 94305 USA

[3] Stanford Univ, Mol Imaging Program Stanford, Dept Bioengn, BioX Program, Stanford, CA 94305 USA

[4] Texas Instruments Inc, Santa Clara, CA 95052 USA

[5] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada

[6] Univ Toronto, Edward S Rogers Sr Dept Elect & Comp Engn, Toronto, ON M5S 3G4, Canada

来源：

BIOMEDICAL OPTICS EXPRESS | 2013年 / 4卷 / 08期

基金：

加拿大自然科学与工程研究理事会;

关键词：

INTEGRATION; LIGHT;

D O I：

10.1364/BOE.4.001332

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Performance improvements in instrumentation for optical imaging have contributed greatly to molecular imaging in living subjects. In order to advance molecular imaging in freely moving, untethered subjects, we designed a miniature vertical-cavity surface-emitting laser (VCSEL)-based biosensor measuring 1cm(3) and weighing 0.7g that accurately detects both fluorophore and tumor-targeted molecular probes in small animals. We integrated a critical enabling component, a complementary metal-oxide semiconductor (CMOS) read-out integrated circuit, which digitized the fluorescence signal to achieve autofluorescence-limited sensitivity. After surgical implantation of the lightweight sensor for two weeks, we obtained continuous and dynamic fluorophore measurements while the subject was un-anesthetized and mobile. The technology demonstrated here represents a critical step in the path toward untethered optical sensing using an integrated optoelectronic implant. (C) 2013 Optical Society of America

引用

页码：1332 / 1341

页数：10