Purification and characterization of beta-D-galactosidase from the pulp of sapota (Achrus sapota) fruit

被引：0
|
作者：
Raju, MRD [1 ]
Kumar, MK [1 ]
机构：
[1] UNIV MYSORE,DEPT STUDIES BIOCHEM,MYSORE 570006,KARNATAKA,INDIA
来源：
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE | 1996年 / 33卷 / 01期
关键词：
beta-galactosidase; sapota; Achrus sapota; pulp; gel filtration; purification; molecular weight; enzyme optima;
D O I：
暂无
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
beta-D-galactosidase (E.C. 3.2. 1.23) from pulp of sapota (Achrus sapota) has been isolated. The enzyme extract was purified by a combination of 80% ammonium sulphate fractionation and combined anion exchange chromatography with DEAE Sephadex A-50 and CM Sephadex C-50. The purified enzyme showed an apparent molecular weight of 120, 200, by gel filtration. The enzyme had an optimum substrate, pH and temperature at 0.5 mM, 5.0 and 50 degrees C, respectively, with p-nitro phenyl - beta-D-galactopyranoside as substrate.
引用
收藏
页码：36 / 40
页数：5
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