Transcutaneous Electrical Nerve Stimulation (TENS) Improves the Diabetic Cytopathy (DCP) via Up-Regulation of CGRP and cAMP

被引:29
作者
Ding, Liucheng [1 ]
Song, Tao [3 ]
Yi, Chaoran [2 ]
Huang, Yi [1 ]
Yu, Wen [2 ]
Ling, Lin [2 ]
Dai, Yutian [2 ]
Wei, Zhongqing [1 ]
机构
[1] Nanjing Med Univ, Affiliated Hosp 2, Dept Urol, Nanjing, Jiangsu, Peoples R China
[2] Nanjing Univ, Sch Med, Drum Tower Hosp, Dept Urol, Nanjing 210008, Jiangsu, Peoples R China
[3] Chinese PLA 454 Hosp, Dept Urol, Nanjing, Jiangsu, Peoples R China
来源
PLOS ONE | 2013年 / 8卷 / 02期
关键词
PRIMARY SENSORY NEURONS; BLADDER DYSFUNCTION; SACRAL NEUROMODULATION; VOIDING DYSFUNCTION; PEPTIDE; RATS; EXPRESSION; DETRUSOR;
D O I
10.1371/journal.pone.0057477
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The objective of this study was to investigate the effects and mechanism of Transcutaneous Electrical Nerve Stimulation (TENS) on the diabetic cytopathy (DCP) in the diabetic bladder. A total of 45 rats were randomly divided into diabetes mellitus (DM)/TENS group (n = 15), DM group (n = 15) and control group (n = 15). The rats in the DM/TENS and TENS groups were electronically stimulated (stimulating parameters: intensity-31 V, frequency-31 Hz, and duration of stimulation of 15 min) for three weeks. Bladder histology, urodynamics and contractile responses to field stimulation and carbachol were determined. The expression of calcitonin gene-related peptide (CGRP) was analyzed by RT-PCR and Western blotting. The results showed that contractile responses of the DM rats were ameliorated after 3 weeks of TENS. Furthermore, TENS significantly increased bladder wet weight, volume threshold for micturition and reduced PVR, V% and cAMP content of the bladder. The mRNA and protein levels of CGRP in dorsal root ganglion (DRG) in the DM/TENS group were higher than those in the DM group. TENS also significantly up-regulated the cAMP content in the bladder body and base compared with diabetic rats. We conclude that TENS can significantly improve the urine contractility and ameliorate the feeling of bladder fullness in DM rats possibly via up-regulation of cAMP and CGRP in DRG.
引用
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页数:7
相关论文
共 27 条
[1]   Calcitonin gene-related peptide decreases expression of acetylcholinesterase in mammalian myotubes [J].
Boudreau-Larivière, C ;
Jasmin, BJ .
FEBS LETTERS, 1999, 444 (01) :22-26
[2]   EFFECT OF SOMATOVISCERAL REFLEXES AND SELECTIVE DERMATOMAL STIMULATION ON POSTCIBAL ANTRAL PRESSURE ACTIVITY [J].
CAMILLERI, M ;
MALAGELADA, JR ;
KAO, PC ;
ZINSMEISTER, AR .
AMERICAN JOURNAL OF PHYSIOLOGY, 1984, 247 (06) :G703-G708
[3]   Time dependent changes in diabetic cystopathy in rats include compensated and decompensated bladder function [J].
Daneshgari, Firouz ;
Liu, Guiming ;
Imrey, Peter B. .
JOURNAL OF UROLOGY, 2006, 176 (01) :380-386
[4]   ELECTRICAL-STIMULATION IN INTERSTITIAL CYSTITIS [J].
FALL, M ;
CARLSSON, CA ;
ERLANDSON, BE .
JOURNAL OF UROLOGY, 1980, 123 (02) :192-195
[5]   Contemporary role of suprapubic cystostomy in treatment of neuropathic bladder dysfunction in spinal cord injured patients [J].
Feifer, Andrew ;
Corcos, Jacques .
NEUROUROLOGY AND URODYNAMICS, 2008, 27 (06) :475-479
[6]   Intravesical electrical stimulation in the treatment of micturition dysfunction in children [J].
Gladh, G ;
Mattsson, S ;
Lindström, S .
NEUROUROLOGY AND URODYNAMICS, 2003, 22 (03) :233-242
[7]   Conditional and Continuous Electrical Stimulation Increase Cystometric Capacity in Persons With Spinal Cord Injury [J].
Horvath, Eric E. ;
Yoo, Paul B. ;
Amundsen, Cindy L. ;
Webster, George D. ;
Grill, Warren M. .
NEUROUROLOGY AND URODYNAMICS, 2010, 29 (03) :401-407
[8]   Electrical stimulation for the treatment of bladder dysfunction: Current status and future possibilities [J].
Jezernik, S ;
Craggs, M ;
Grill, WM ;
Creasey, G ;
Rijkhoff, NJM .
NEUROLOGICAL RESEARCH, 2002, 24 (05) :413-430
[9]   Sacral neuromodulation for urinary retention [J].
Kessler, Thomas M. ;
Fowler, Clare J. .
NATURE CLINICAL PRACTICE UROLOGY, 2008, 5 (12) :657-666
[10]   Functional, morphological and molecular characterization of bladder dysfunction in streptozotocin-induced diabetic mice: evidence of a role for L-type voltage-operated Ca2+ channels [J].
Leiria, L. O. S. ;
Monica, F. Z. T. ;
Carvalho, F. D. G. F. ;
Claudino, M. A. ;
Franco-Penteado, C. F. ;
Schenka, A. ;
Grant, A. D. ;
De Nucci, G. ;
Antunes, E. .
BRITISH JOURNAL OF PHARMACOLOGY, 2011, 163 (06) :1276-1288