Biomarkers associated with low, moderate, and high vastus lateralis muscle hypertrophy following 12 weeks of resistance training

被引:77
作者
Mobley, Christopher B. [1 ]
Haun, Cody T. [1 ]
Roberson, Paul A. [1 ]
Mumford, Petey W. [1 ]
Kephart, Wesley C. [1 ]
Romero, Matthew A. [1 ]
Osburn, Shelby C. [1 ]
Vann, Christopher G. [1 ]
Young, Kaelin C. [1 ,2 ]
Beck, Darren T. [1 ,2 ]
Martin, Jeffrey S. [1 ,2 ]
Lockwood, Christopher M. [3 ]
Roberts, Michael D. [1 ,2 ]
机构
[1] Auburn Univ, Sch Kinesiol, Auburn, AL 36849 USA
[2] Edward Via Coll Osteopath Med, Dept Cell Biol & Physiol, Auburn Campus, Auburn, AL 36832 USA
[3] Lockwood LLC, Draper, UT USA
关键词
SKELETAL-MUSCLE; MYOFIBER HYPERTROPHY; RIBOSOME BIOGENESIS; PROTEIN-SYNTHESIS; GENE-EXPRESSION; MYONUCLEAR ADDITION; EXERCISE; MYOSTATIN; TESTOSTERONE; HUMANS;
D O I
10.1371/journal.pone.0195203
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We sought to identify biomarkers which delineated individual hypertrophic responses to resistance training. Untrained, college-aged males engaged in full-body resistance training (3 d/wk) for 12 weeks. Body composition via dual x-ray absorptiometry (DXA), vastus lateralis (VL) thickness via ultrasound, blood, VL muscle biopsies, and three-repetition maximum (3-RM) squat strength were obtained prior to (PRE) and following (POST) 12 weeks of training. K-means cluster analysis based on VL thickness changes identified LOW [n = 17; change (mean +/- SD) = +0.11 +/- 0.14 cm], modest (MOD; n = 29, +0.40 +/- 0.06 cm), and high (HI; n = 21, +0.69 +/- 0.14 cm) responders. Biomarkers related to histology, ribosome biogenesis, proteolysis, inflammation, and androgen signaling were analyzed between clusters. There were main effects of time (POST>PRE, p<0.05) but no cluster x time interactions for increases in DXA lean body mass, type I and II muscle fiber cross sectional area and myonuclear number, satellite cell number, and macronutrients consumed. Interestingly, PRE VL thickness was similar to 12% greater in LOW versus HI (p = 0.021), despite POST values being similar to 12% greater in HI versus LOW (p = 0.006). However there was only a weak correlation between PRE VL thickness scores and change in VL thickness (r(2) = 0.114, p = 0.005). Forced post hoc analysis indicated that muscle total RNA levels (i.e., ribosome density) did not significantly increase in the LOW cluster (351 +/- 70 ng/mg to 380 +/- 62, p = 0.253), but increased in the MOD (369 +/- 115 to 429 +/- 92, p = 0.009) and HI clusters (356 +/- 77 to 470 +/- 134, p<0.001; POST HI>POST LOW, p = 0.013). Nonetheless, there was only a weak association between change in muscle total RNA and VL thickness (r(2) = 0.079, p = 0.026). IL-1 beta mRNA levels decreased in the MOD and HI clusters following training (p<0.05), although associations between this marker and VL thickness changes were not significant (r(2) = 0.0002, p = 0.919). In conclusion, individuals with lower pre-training VL thickness values and greater increases muscle total RNA levels following 12 weeks of resistance training experienced greater VL muscle growth, although these biomarkers individually explained only similar to 8-11% of the variance in hypertrophy.
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页数:20
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