Differentiation of Reactive and Tumor Metastatic Lymph Nodes with Diffusion-weighted and SPIO-Enhanced MRI

被引:49
作者
Zhang, Fan [1 ,2 ]
Zhu, Lei [1 ,2 ]
Huang, Xinglu [2 ]
Niu, Gang [2 ]
Chen, Xiaoyuan [2 ]
机构
[1] Xiamen Univ, Sch Publ Hlth, Ctr Mol Imaging & Translat Med, Xiamen 361000, Peoples R China
[2] NIBIB, LOMIN, NIH, Bethesda, MD 20892 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
Lymph node metastasis; Diffusion-weighted imaging (DWI); Superparamagnetic iron oxide (SPIO); Magnetic resonance imaging (MRI); Apparent diffusion coefficient (ADC) map; SUPERPARAMAGNETIC IRON-OXIDE; SQUAMOUS-CELL CARCINOMA; WHOLE-BODY; CERVICAL LYMPHADENOPATHY; POTENTIAL APPLICATIONS; DIAGNOSTIC-ACCURACY; PROSTATE-CANCER; NECK; LYMPHOGRAPHY; HEAD;
D O I
10.1007/s11307-012-0562-2
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Determination of lymphatic metastasis is of great importance for both treatment planning and patient prognosis. We aim to distinguish tumor metastatic lymph nodes (TLNs) and reactive lymph nodes (RLNs) with diffusion-weighted and superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance imaging (MRI). Ipsilateral popliteal lymph node metastasis or lymphadenitis model was established by hock injection of either luciferase-expressing 4T1 murine breast cancer cells or complete Freund's adjuvant in male BALB/c mice. At different time points after inoculation, bioluminescence imaging and T-2-weighted, diffusion-weighted, and SPIO-enhanced MRI were performed. Imaging findings were confirmed by histopathological staining. Size enlargement was observed in both TLNs and RLNs. At day 28, TLNs showed strong bioluminescence signal and bigger size than RLNs (p < 0.01). At early stages up to day 21, both TLNs and RLNs appeared homogeneous on diffusion-weighted imaging. At day 28, TLNs showed heterogeneous apparent diffusion coefficient (ADC) map with significantly higher average ADC value of 0.41 +/- 0.03 x 10(-3)mm(2)/s than that of RLNs (0.34 +/- 0.02 x 10(-3)mm(2)/s; p < 0.05). On SPIO-enhanced MRI, both TLNs and RLNs showed distinct T-2 signal reduction at day 21 after inoculation. At day 28, TLNs demonstrated partial uptake of the iron oxide particles, which was confirmed by Prussian blue staining. Both diffusion-weighted and SPIO-enhanced MRI can distinguish tumor metastatic lymph nodes from reactive lymph nodes. However, neither method is able to detect tumor metastasis to the draining lymph nodes at early stages.
引用
收藏
页码:40 / 47
页数:8
相关论文
共 35 条
  • [1] Evaluation of neck and body metastases to nodes with ferumoxtran 10-enhanced MR imaging: Phase III safety and efficacy study
    Anzai, Y
    Piccoli, CW
    Outwater, EK
    Stanford, W
    Bluemke, DA
    Nurenberg, P
    Saini, S
    Maravilla, KR
    Feldman, DE
    Schmiedl, UP
    Brunberg, JA
    Francis, IR
    Harms, SE
    Som, PM
    Tempany, CM
    [J]. RADIOLOGY, 2003, 228 (03) : 777 - 788
  • [2] ASLAKSON CJ, 1992, CANCER RES, V52, P1399
  • [3] Diagnostic accuracy and additional value of diffusion-weighted imaging for discrimination of malignant cervical lymph nodes in head and neck squamous cell carcinoma
    de Bondt, R. B. J.
    Hoeberigs, M. C.
    Nelemans, P. J.
    Deserno, W. M. L. L. G.
    Peutz-Kootstra, C.
    Kremer, B.
    Beets-Tan, R. G. H.
    [J]. NEURORADIOLOGY, 2009, 51 (03) : 183 - 192
  • [4] Reducing motion artefacts in diffusion-weighted MRI of the brain: efficacy of navigator echo correction and pulse triggering
    Dietrich, O
    Heiland, S
    Benner, T
    Sartor, K
    [J]. NEURORADIOLOGY, 2000, 42 (02) : 85 - 91
  • [5] Preliminary Results for Characterization of Pelvic Lymph Nodes in Patients With Prostate Cancer by Diffusion-Weighted MR-Imaging
    Eiber, Matthias
    Beer, Ambros J.
    Holzapfel, Kionstantin
    Tauber, Robert
    Ganter, Carl
    Weirich, Gregor
    Krause, Bernd J.
    Rummeny, Ernst J.
    Gaa, Jochen
    [J]. INVESTIGATIVE RADIOLOGY, 2010, 45 (01) : 15 - 23
  • [6] Noninvasive detection of clinically occult lymph-node metastases in prostate cancer
    Harisinghani, MG
    Barentsz, J
    Hahn, PF
    Deserno, WM
    Tabatabaei, S
    van de Kaa, CH
    de la Rosette, J
    Weissleder, R
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (25) : 2491 - U5
  • [7] Interstitial MR lymphography with MS-325: Characterization of normal and tumor-invaded lymph nodes in a rabbit model
    Herborn, CU
    Lauenstein, TC
    Vogt, FM
    Lauffer, RB
    Debatin, JF
    Ruehm, SG
    [J]. AMERICAN JOURNAL OF ROENTGENOLOGY, 2002, 179 (06) : 1567 - 1572
  • [8] Value of diffusion-weighted MR imaging in the differentiation between benign and malignant cervical lymph nodes
    Holzapfel, Konstantin
    Duetsch, Sabrina
    Fauser, Claudius
    Eiber, Matthias
    Rummeny, Ernst J.
    Gaa, Jochen
    [J]. EUROPEAN JOURNAL OF RADIOLOGY, 2009, 72 (03) : 381 - 387
  • [9] Improving the Magnetic Resonance Imaging Contrast and Detection Methods with Engineered Magnetic Nanoparticles
    Huang, Jing
    Zhong, Xiaodong
    Wang, Liya
    Yang, Lily
    Mao, Hui
    [J]. THERANOSTICS, 2012, 2 (01): : 86 - 102
  • [10] Feasibility of diffusion-weighted imaging in the differentiation of metastatic from nonmetastatic lymph nodes: Early experience
    Kim, Jeong Kon
    Kim, Kyoung Ah
    Park, Bum-Woo
    Kim, Namkug
    Cho, Kyoung-Sik
    [J]. JOURNAL OF MAGNETIC RESONANCE IMAGING, 2008, 28 (03) : 714 - 719