Novel Mononuclear Ruthenium(II) Compounds in Cancer Therapy

The present study was conducted to evaluate in vivo anticancer activity of two novel mononuclear ruthenium(II) compounds, namely Ru(1,10-phenanthroline)(2)(2-nitro phenyl thiosemicarbazone)Cl-2 (Compound R-1) and Ru (1,10-phenanthroline)(2)(2-hydroxy phenyl thiosemicarbazone)Cl-2 (Compound R-2) against Ehrlich ascites carcinoma (EAC) mice and in vitro cytotoxic activity against IEC-6 (small intestine) cell lines and Artemia salina nauplii using MTT [(3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide)] and BLT [brine shrimp lethality] assays respectively. The tested ruthenium compounds at the doses 2 and 4 mg/kg body weight showed promising biological activity especially in decreasing the tumor volume, viable ascites cell counts and body weights. These compounds prolonged the life span(% ILS), mean survival time (MST) of mice bearing-EAC tumor. The results for in vitro cytotoxicity against IEC-6 cells showed the ruthenium compound R-2 to have significant cytotoxic activity with a IC50 value of 20.0 mu g/mL than R-1 (IC50=78.8 mu g/mL) in the MTT assay and the LC50 values of R-1 and R-2 compounds were found to be 38.3 and 43.8 mu g/mL respectively in the BLT assay. The biochemical and histopathological results revealed that there was no significant hepatotoxicity and nephrotoxicity associated with the ruthenium administration to mice.