Impact of copper on the abundance and diversity of sulfate-reducing prokaryotes in two chilean marine sediments

被引：11

作者：

Besaury, Ludovic ^{[1
,2
]}

Ouddane, Baghdad ^{[3
]}

Pavissich, Juan Pablo ^{[4
]}

Dubrulle-Brunaud, Carole ^{[2
]}

Gonzalez, Bernardo ^{[5
]}

Quillet, Laurent ^{[1
]}

机构：

[1] Univ Rouen, Lab Univ MEBE Microbiol Environm & Biol Evolut, F-76821 Mont St Aignan, France

[2] Univ Rouen, CNRS, UMR 6143 M2C, Fac Sci,Grp Microbiol, F-76821 Mont St Aignan, France

[3] Univ Lille 1, UMR 8217, Equipe Chim Analyt & Marine, F-59655 Villeneuve Dascq, France

[4] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Santiago, Chile

[5] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Santiago, Chile

来源：

MARINE POLLUTION BULLETIN | 2012年 / 64卷 / 10期

关键词：

Marine sediment; Copper contamination; Sulfate-reducing prokaryotes; Real-time PCR; DGGE; Phylogenetic analysis; SULFITE REDUCTASE GENES; 16S RIBOSOMAL-RNA; METAL CONTAMINATION GRADIENT; GEL-ELECTROPHORESIS ANALYSIS; ACID-MINE DRAINAGE; REAL-TIME PCR; ESTUARINE SEDIMENTS; BACTERIAL COMMUNITIES; MICROBIAL COMMUNITIES; HEAVY-METALS;

D O I：

10.1016/j.marpolbul.2012.07.042

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We studied the abundance and diversity of the sulfate-reducing prokaryotes (SRPs) in two 30-cm marine chilean sediment cores, one with a long-term exposure to copper-mining residues, the other being a non-exposed reference sediment. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene beta-subunit (dsrB) and showed that SRPs are sensitive to high copper concentrations, as the mean number of SRPs all along the contaminated sediment was two orders of magnitude lower than in the reference sediment. SRP diversity was analyzed by using the dsrB-sequences-based PCR-DGGE method and constructing gene libraries for dsrB-sequences. Surprisingly, the diversity was comparable in both sediments, with dsrB sequences belonging to Desulfobacteraceae, Syntrophobacteraceae, and Desulfobulbaceae, SRP families previously described in marine sediments, and to a deep branching dsrAB lineage. The hypothesis of the presence of horizontal transfer of copper resistance genes in the microbial population of the polluted sediment is discussed. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：2135 / 2145

页数：11

共 100 条

[1] Genome Sequence of Desulfosporosinus sp OT, an Acidophilic Sulfate-Reducing Bacterium from Copper Mining Waste in Norilsk, Northern Siberia [J].