Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer

被引：85
|
作者：
Kim, Gae Baik [1 ]
Kim, Young-Pil [1 ]
机构：
[1] Hanyang Univ, Dept Life Sci, Seoul 133791, South Korea
来源：
THERANOSTICS | 2012年 / 2卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
protease; quantum dot; energy transfer; BRET; FRET; multiplex; nanoparticle; luciferase; quencher; MATRIX-METALLOPROTEINASE INHIBITORS; SEMICONDUCTOR NANOCRYSTALS; GOLD NANOPARTICLES; CANCER-THERAPY; PROTEOLYTIC ACTIVITY; FUTURE-PROSPECTS; SERUM-LEVELS; FLUORESCENCE; FRET; ASSAY;
D O I：
10.7150/thno.3476
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
This review demonstrates the detection of protease activity based on the energy transfer of quantum dots (QDs). By incorporation of varying protease substrates into designed QD probes both in fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) system, proteolytic activity led to changes in the energy transfer efficiency. Especially due to the superior properties of QDs, it can be served as an excellent probe for a multiplexed and high-throughput protease assay with high sensitivity. It is anticipated that the QD-based FRET/BRET probes will have a great potential for dissecting the fundamental roles of proteases and designing potential protease inhibitors as therapeutic drugs in biology and nanomedicine.
引用
收藏
页码：127 / 138
页数：12
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