Overexpression of IL-6 by Gene Transfer Stimulates IL-8-mediated Invasiveness of KYSE170 Esophageal Carcinoma Cells

被引:0
|
作者
Okamura, Shinichi [1 ]
Fujiwara, Hitoshi [1 ]
Yoneda, Masayuki [1 ]
Furutani, Akinobu [1 ]
Todo, Momoko [1 ]
Ikai, Atsushi [1 ]
Tada, Hiroyuki [1 ]
Okamura, Hiroko [1 ]
Umehara, Seiji [1 ]
Shiozaki, Atsushi [1 ]
Kubota, Takeshi [1 ]
Komatsu, Shuhei [1 ]
Ichikawa, Daisuke [1 ]
Okamoto, Kazuma [1 ]
Ochiai, Toshiya [1 ]
Otsuji, Eigo [1 ]
机构
[1] Kyoto Prefectural Univ Med, Dept Surg, Div Digest Surg, Kamigyo Ku, Kyoto 602, Japan
关键词
Esophageal carcinoma; IL-6; IL-8; invasiveness; KYSE170; cells; BREAST-CANCER CELLS; GROWTH-FACTOR RECEPTOR; NEOADJUVANT CHEMORADIOTHERAPY; SERUM-LEVELS; INTERLEUKIN-6; SURVIVAL; PROLIFERATION; INFLAMMATION; EXPRESSION; MIGRATION;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Interleukin-6 (IL-6) has been associated with disease progression and poor prognosis in esophageal carcinoma. The aim of this study was to investigate the possible influence of IL-6 on the biological activities of esophageal carcinoma cells in terms of invasiveness. The human esophageal carcinoma cell line, KYSE170, was transfected with a plasmid vector expressing IL-6, and a stable transfectant overexpressing IL-6 was established. Invasiveness was evaluated by an invasion assay and compared between IL-6 and control transfectants. The invasiveness of the IL-6 transfectant was significantly higher than that of the control transfectant, and was significantly reduced by IL-6-specific siRNA. In reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, IL-8 expression was significantly higher in the IL-6 transfectant than in the control transfectant, whereas the expression of Hepatocyte growth factor (HGF) and Vascular endothelial growth factor (VEGF) was not different. IL-8 expression in the IL-6 transfectant was significantly inhibited by IL-8-specific siRNA, whereas IL-6 expression was not. In addition, the invasiveness of the IL-6 transfectant was significantly reduced by IL-8-specific siRNA. These results indicate that the overexpression of IL-6 increases the invasiveness of KYSE170 esophageal carcinoma cells and IL-6-induced IL-8 plays a predominant role in increasing invasiveness.
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收藏
页码:1483 / 1489
页数:7
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