Dissemination of blaVIM in Greece at the peak of the epidemic of 2005-2006: clonal expansion of Klebsiella pneumoniae clonal complex 147

被引：31

作者：

Hasan, C. M. ^{[1
]}

Turlej-Rogacka, A. ^{[1
]}

Vatopoulos, A. C. ^{[2
,3
]}

Giakkoupi, P. ^{[2
,3
]}

Maatallah, M. ^{[4
]}

Giske, C. G. ^{[1
]}

机构：

[1] Karolinska Inst, Karolinska Univ Hosp, MTC, SE-17176 Stockholm, Sweden

[2] KEELNPO, Natl Sch Publ Hlth, Dept Microbiol, Athens, Greece

[3] KEELNPO, Cent Publ Hlth Lab, Athens, Greece

[4] Fac Pharm, Lab Anal Traitement & Valorisat Polluants Environ, Monastir, Tunisia

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2014年 / 20卷 / 01期

关键词：

MLST; clonal complex; metallo--lactamase; AmpC; plasmid replicon; BETA-LACTAMASE; EMERGENCE; PCR; ENTEROBACTERIACEAE; INDIA; KPC-2; GENE;

D O I：

10.1111/1469-0691.12187

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

VIM-producing Klebsiella pneumoniae (n=21) isolated from ten Greek hospitals during 2003-2007 were characterized with multilocus sequence typing (MLST), semi-automated repetitive sequence-based PCR (rep-PCR) (Diversilab), plasmid replicon typing, serotyping and screening for multiple resistance determinants. The isolates were selected to represent different strain clusters (defined by >80% similarity) according to pulsed-field gel electrophoresis. MLST identified three major clonal complexes (CCs); CC147 (n=8), CC18 (n=5) and CC14 (n=3). Plasmid replicon typing showed that IncA/C and/or IncFII(K) replicons were detected among isolates in each of the major CCs. Good concordance was observed between semi-automated-rep PCR genotyping and MLST.

引用

页码：34 / 37

页数：4

共 15 条

[1] Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases [J].

Birkett, Christopher I. ;

Ludlam, Hugo A. ;

Woodford, Neil ;

Brown, Derek F. J. ;

Brown, Nicholas M. ;

Roberts, Mark T. M. ;

Milner, Nicola ;

Curran, Martin D. .

JOURNAL OF MEDICAL MICROBIOLOGY, 2007, 56 (01) :52-55

[2] Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium [J].

Bogaerts, Pierre ;

Galimand, Marc ;

Bauraing, Caroline ;

Deplano, Ariane ;

Vanhoof, Raymond ;

De Mendonca, Ricardo ;

Rodriguez-Villalobos, Hector ;

Struelens, Marc ;

Glupczynski, Youri .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 59 (03) :459-464

[3] Development of a real-time SYBRGreen PCR assay for rapid detection of acquired AmpC in Enterobacteriaceae (vol 82, pg 229, 2010) [J].

Brolund, Alma ;

Wisell, Karin Tegmark ;

Edquist, Petra J. ;

Elfstrom, Lisbeth ;

Walder, Mats ;

Giske, Christian G. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2012, 88 (01) :188-188

[4] Identification of plasmids by PCR-based replicon typing [J].

Carattoli, A ;

Bertini, A ;

Villa, L ;

Falbo, V ;

Hopkins, KL ;

Threlfall, EJ .

JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 63 (03) :219-228

[5] Appearance of aac(6′)-lb-cr gene among extended-spectrum β-lactamase-producing Enterobacteriaceae in a French hospital [J].

Fihman, V. ;

Lartigue, M. F. ;

Jacquier, H. ;

Meunier, F. ;

Schnepf, N. ;

Raskine, L. ;

Riahi, J. ;

Pors, M. -J. Sanson-le ;

Bercot, B. .

JOURNAL OF INFECTION, 2008, 56 (06) :454-459

[6] VIM-1 metallo-β-lactamase-producing Klebsiella pneumoniae strains in Greek hospitals [J].

Giakkoupi, P ;

Xanthaki, A ;

Kanelopoulou, M ;

Vlahaki, A ;

Miriagou, V ;

Kontou, S ;

Papafraggas, E ;

Malamou-Lada, H ;

Tzouvelekis, LS ;

Legakis, NJ ;

Vatopoulos, AC .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (08) :3893-3896

[7] Emerging Klebsiella pneumoniae Isolates Coproducing KPC-2 and VIM-1 Carbapenemases [J].

Giakkoupi, Panagiota ;

Pappa, Olga ;

Polemis, Michalis ;

Vatopoulos, Alkiviadis C. ;

Miriagou, Vivi ;

Zioga, Anastasia ;

Papagiannitsis, Costas C. ;

Tzouvelekis, Leonidas S. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (09) :4048-4050

[8] Diverse Sequence Types of Klebsiella pneumoniae Contribute to the Dissemination of blaNDM-1 in India, Sweden, and the United Kingdom [J].

Giske, Christian G. ;

Froding, Inga ;

Hasan, Chowdhury Mehedi ;

Turlej-Rogacka, Agata ;

Toleman, Mark ;

Livermore, David ;

Woodford, Neil ;

Walsh, Timothy R. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (05) :2735-2738

[9] Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study [J].

Kumarasamy, Karthikeyan K. ;

Toleman, Mark A. ;

Walsh, Timothy R. ;

Bagaria, Jay ;

Butt, Fafhana ;

Balakrishnan, Ravikumar ;

Chaudhary, Uma ;

Doumith, Michel ;

Giske, Christian G. ;

Irfan, Seema ;

Krishnan, Padma ;

Kumar, Anil V. ;

Maharjan, Sunil ;

Mushtaq, Shazad ;

Noorie, Tabassum ;

Paterson, David L. ;

Pearson, Andrew ;

Perry, Claire ;

Pike, Rachel ;

Rao, Bhargavi ;

Ray, Ujjwayini ;

Sarma, Jayanta B. ;

Sharma, Madhu ;

Sheridan, Elizabeth ;

Thirunarayan, Mandayam A. ;

Turton, Jane ;

Upadhyay, Supriya ;

Warner, Marina ;

Welfare, William ;

Livermore, David M. ;

Woodford, Neil .

LANCET INFECTIOUS DISEASES, 2010, 10 (09) :597-602

[10] Emergence of Escherichia coli sequence type 410 (ST410) with KPC-2 β-lactamase [J].

Mavroidi, Angeliki ;

Miriagou, Vivi ;

Malli, Ergina ;

Stefos, Angelos ;

Dalekos, George N. ;

Tzouvelekis, Leonidas S. ;

Petinaki, Efthymia .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2012, 39 (03) :247-250

← 1 2 →