Subangstrom single-molecule measurements of motor proteins using a nanopore

被引：104

作者：

Derrington, Ian M. ^{[1
]}

Craig, Jonathan M. ^{[1
]}

Stava, Eric ^{[2
]}

Laszlo, Andrew H. ^{[1
]}

Ross, Brian C. ^{[1
]}

Brinkerhoff, Henry ^{[1
]}

Nova, Ian C. ^{[1
]}

Doering, Kenji ^{[1
]}

Tickman, Benjamin I. ^{[1
]}

Ronaghi, Mostafa ^{[2
]}

Mandell, Jeffrey G. ^{[2
]}

Gunderson, Kevin L. ^{[2
]}

Gundlach, Jens H. ^{[1
]}

机构：

[1] Univ Washington, Dept Phys, Seattle, WA 98195 USA

[2] Illumina Inc, San Diego, CA USA

来源：

NATURE BIOTECHNOLOGY | 2015年 / 33卷 / 10期

基金：

美国国家卫生研究院;

关键词：

STRANDED-DNA; MSPA; POLYMERASE; HELICASE;

D O I：

10.1038/nbt.3357

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of similar to 300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to similar to 40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.

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页码：1073 / +

页数：4

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