Subangstrom single-molecule measurements of motor proteins using a nanopore

被引：101

作者：

Derrington, Ian M. ^{[1
]}

Craig, Jonathan M. ^{[1
]}

Stava, Eric ^{[2
]}

Laszlo, Andrew H. ^{[1
]}

Ross, Brian C. ^{[1
]}

Brinkerhoff, Henry ^{[1
]}

Nova, Ian C. ^{[1
]}

Doering, Kenji ^{[1
]}

Tickman, Benjamin I. ^{[1
]}

Ronaghi, Mostafa ^{[2
]}

Mandell, Jeffrey G. ^{[2
]}

Gunderson, Kevin L. ^{[2
]}

Gundlach, Jens H. ^{[1
]}

机构：

[1] Univ Washington, Dept Phys, Seattle, WA 98195 USA

[2] Illumina Inc, San Diego, CA USA

来源：

NATURE BIOTECHNOLOGY | 2015年 / 33卷 / 10期

基金：

美国国家卫生研究院;

关键词：

STRANDED-DNA; MSPA; POLYMERASE; HELICASE;

D O I：

10.1038/nbt.3357

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of similar to 300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to similar to 40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.

引用

页码：1073 / +

页数：4

共 19 条

[1] Adam G., 1968, Structural Chemistry and Molecular Biology, V198, P198
[2] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules
Akeson, M
Branton, D
Kasianowicz, JJ
Brandin, E
Deamer, DW
[J]. BIOPHYSICAL JOURNAL, 1999, 77 (06) : 3227 - 3233
[3] Elastic properties and secondary structure formation of single-stranded DNA at monovalent and divalent salt conditions
Bosco, Alessandro
Camunas-Soler, Joan
Ritort, Felix
[J]. NUCLEIC ACIDS RESEARCH, 2014, 42 (03) : 2064 - 2074
[4] Structural basis for DNA duplex separation by a superfamily-2 helicase
Buettner, Katharina
Nehring, Sebastian
Hopfner, Karl-Peter
[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (07) : 647 - 652
[5] Single-molecule DNA detection with an engineered MspA protein nanopore
Butler, Tom Z.
Pavlenok, Mikhail
Derrington, Ian M.
Niederweis, Michael
Gundlach, Jens H.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (52) : 20647 - 20652
[6] Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision
Cherf, Gerald M.
Lieberman, Kate R.
Rashid, Hytham
Lam, Christopher E.
Karplus, Kevin
Akeson, Mark
[J]. NATURE BIOTECHNOLOGY, 2012, 30 (04) : 344 - 348
[7] Nanopore DNA sequencing with MspA
Derrington, Ian M.
Butler, Tom Z.
Collins, Marcus D.
Manrao, Elizabeth
Pavlenok, Mikhail
Niederweis, Michael
Gundlach, Jens H.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (37) : 16060 - 16065
[8] Single-Molecule Views of Protein Movement on Single-Stranded DNA
Ha, Taekjip
Kozlov, Alexander G.
Lohman, Timothy M.
[J]. ANNUAL REVIEW OF BIOPHYSICS, VOL 41, 2012, 41 : 295 - 319
[9] Driven DNA transport into an asymmetric nanometer-scale pore
Henrickson, SE
Misakian, M
Robertson, B
Kasianowicz, JJ
[J]. PHYSICAL REVIEW LETTERS, 2000, 85 (14) : 3057 - 3060
[10] Single-molecule studies of RNA polymerase: Motoring along
Herbert, Kristina M.
Greenleaf, William J.
Block, Steven M.
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 2008, 77 : 149 - 176

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