Phenolic derivatives in raspberry (Rubus L.) germplasm collection in Bulgaria

被引:20
作者
Gevrenova, Reneta [1 ]
Badjakov, Ilian [2 ]
Nikolova, Milena [3 ]
Doichinova, Irini [4 ]
机构
[1] Med Univ Sofia, Fac Pharm, Dept Pharmacognosy, Sofia 1000, Bulgaria
[2] AgroBio Inst, Sofia 1164, Bulgaria
[3] Inst Biodivers & Ecosyst Res, Sofia 1113, Bulgaria
[4] Med Univ Sofia, Fac Pharm, Dept Chem, Sofia 1000, Bulgaria
关键词
Raspberry; Phenolic acids; Flavonoids; Caffeic acid; PCA; Hierarchical clustering; LEAVES; FLAVONOIDS; IDAEUS; ACID;
D O I
10.1016/j.bse.2013.06.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Leaves of 22 accessions of the Bulgarian raspberry (Rubus L) germplasm collection - five Bulgarian and seven foreign cultivars, eight elite clones and two wild species, Rubus occidentalis L. and Rubus odoratus L, were analysed for phenolic constituents. The quantitative determination of caffeic (1), p-coumaric (2) and ferulic (3) acids, hyperoside (4), tiliroside (5), and isoquercitrin (6) was performed by RP-HPLC using linear gradient elution and UV detection at 254 and 310 nm. The detection limits ranged from 0.23 mu g/ml (4) to 0.55 mu g/ml (2). Caffeic acid was the dominant phenolic acid in the majority of the samples being present in amounts between 0.05 +/- 0.01 mg/g dry weight in R. occidentalis and 1.43 +/- 0.06 mg/g in the cultivars. The highest content of flavonols was found in the Bulgarian raspberry elite clones: 1.70 +/- 0.002 mg/g (6), 0.60 +/- 0.004 mg/g (5) and 0.97 0.01 mg/g (4). Data were analyzed by hierarchical clustering (HC) and principal component analysis (PCA). The HC and PCA scoring plot showed that the samples could be classified into three clusters. Cluster Cl comprised cultivars characterized by high content of phenolic acids (1-3). Seven cultivars and the wild species R. occidentalis formed the cluster C2 presenting low content of phenolic compounds. Cluster C3 grouped the Bulgarian raspberry elite clones and R. odoratus sharing the highest content of flavonols (5 and 6). The Bulgarian elite clone E23617 displayed the highest content of the studied phenolic derivatives. The PCA loading plot showed that 1 can be used to distinguish between different raspberry varieties. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:419 / 427
页数:9
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