Hyperbaric oxygen stimulates vascularization and bone formation in rat calvarial defects

被引:26
作者
Pedersen, T. O. [1 ,2 ]
Xing, Z. [1 ]
Finne-Wistrand, A. [3 ]
Hellem, S. [1 ,4 ]
Mustafa, K. [1 ]
机构
[1] Univ Bergen, Dept Clin Dent, Ctr Clin Dent Res, N-5009 Bergen, Norway
[2] Univ Bergen, Dept Biomed, N-5009 Bergen, Norway
[3] KTH Royal Inst Technol, Dept Fibre & Polymer Technol, Stockholm, Sweden
[4] Univ Bergen, Dept Clin Dent, Sect Oral & Maxillofacial Surg, N-5009 Bergen, Norway
关键词
hyperbaric oxygen; vascularization; copolymer scaffold; osteogenesis; osteoprogenitor cells; STEM-CELL MOBILIZATION; DECREASES INFLAMMATION; ANIMAL-MODEL; HYPEROXIA; THERAPY; DIFFERENTIATION; REGENERATION;
D O I
10.1016/j.ijom.2013.01.003
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Hyperbaric oxygen (HBO) therapy is used to treat or prevent tissue necrosis in patients undergoing irradiation. Many such patients require reconstructive surgery, but little is known of the effects of 030 on bone vascularization and regeneration. In this study, copolymer poly(L-lactide-co-1,5-dioxepan-2-one) (poly(LLA-co-DXO )) scaffolds were implanted into critical-sized calvarial defects in Wistar rats. The animals were randomly allotted to hyperbaric or normobaric gxygen groups. The treatment group received five sessions weekly for 90 min at increased atmospheric pressure, for up to 4 weeks. Samples were retrieved at weeks 2 and 8, i.e. after a total of 10 and 20 sessions, respectively. The samples were analyzed by real-time reverse transcriptase polymerase chain reaction (RT-PCR) and histology at week 2, and radiographically and histologically at week 8. At week 2, defects treated with HBO exhibited greater numbers of cells positive for the endothelial marker CD31, up-regulated gene expression of osteogenic markers, and down-regulated expression of pro-inflammatory cytokines. At week 8, radiographic examination revealed that calvarial defects subjected to HBO exhibited a higher percentage of radiopacities than normobaric controls, and histological examination disclosed enhanced bone healing. These results confirmed that HBO treatment was effective in stimulating vascularization and bone formation in rat calvarial defects.
引用
收藏
页码:907 / 914
页数:8
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