Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

被引：12

作者：

Henares, Terence G. ^{[1
]}

Uenoyama, Yuta ^{[1
]}

Nogawa, Yuto ^{[1
]}

Ikegami, Ken ^{[2
]}

Citterio, Daniel ^{[2
]}

Suzuki, Koji ^{[2
]}

Funano, Shun-ichi ^{[1
]}

Sueyoshi, Kenji ^{[1
]}

Endo, Tatsuro ^{[1
]}

Hisamoto, Hideaki ^{[1
]}

机构：

[1] Osaka Prefecture Univ, Grad Sch Engn, Naka Ku, Sakai, Osaka 5998531, Japan

[2] Keio Univ, Fac Sci & Technol, Tokyo, Kanagawa 108, Japan

来源：

ANALYST | 2013年 / 138卷 / 11期

关键词：

RHODAMINE DERIVATIVES; FLUOROGENIC PROBE; QUANTUM YIELDS; ANTIBODIES;

D O I：

10.1039/c3an00098b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.

引用

页码：3139 / 3141

页数：3

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