Determination of γ-Aminobutyric Acid in Leaf, Stem and Root Bark of Mulberry by High-Performance Anion Exchange Chromatography-Integrated Pulsed Amperometric Detection

被引：1
作者：
Kwon, H. -J. [1 ]
Kim, S. -H. [1 ]
Hwang, J. -H. [1 ]
Park, Y. -D. [1 ]
机构：
[1] Kyung Hee Univ, Grad Sch, Dept Prevent & Social Dent, Seoul 130701, South Korea
来源：
ACTA CHROMATOGRAPHICA | 2013年 / 25卷 / 03期
关键词：
gamma-aminobutyric acid; mulberry; anion-exchange chromatography; integrated pulsed amperometric detection; LIQUID-CHROMATOGRAPHY; AMINO-ACIDS; GABA; GLUTAMATE; SECRETION;
D O I：
10.1556/AChrom.25.2013.3.13
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple high-performance anion-exchange chromatography-integrated pulsed amperometric detection method was developed to determine the gamma-aminobutyric acid (GABA) content in mulberry. GABA was separated in anion-exchange column under isocratic condition of 20 mM sodium hydroxide solution and detected at integrated pulsed amperometric detection at pico-gram levels. The contents of GABA in the leaf, stem, and root bark of mulberry were calculated without any derivatization or sample purification process using this method. This method showed good linearity, precision, and accuracy for GABA analysis.
引用
收藏
页码：581 / 588
页数：8
相关论文
共 16 条
  • [1] Bang H. S., 1998, KOREAN J SERIC SCI, V40, P13
  • [2] INVOLVEMENT OF THE AMYGDALA GABAERGIC SYSTEM IN THE MODULATION OF MEMORY STORAGE
    BRIONI, JD
    NAGAHARA, AH
    MCGAUGH, JL
    [J]. BRAIN RESEARCH, 1989, 487 (01) : 105 - 112
  • [3] Rapid analysis of GABA and glutamate in microdialysis samples using high performance liquid chromatography and tandem mass spectrometry
    Buck, Kerstin
    Voehringer, Patrizia
    Ferger, Boris
    [J]. JOURNAL OF NEUROSCIENCE METHODS, 2009, 182 (01) : 78 - 84
  • [4] An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain
    Clarke, Gerard
    Mahony, Siobhain
    Malone, Gwen
    Dinan, Timothy G.
    [J]. JOURNAL OF NEUROSCIENCE METHODS, 2007, 160 (02) : 223 - 230
  • [5] Pivagabine decreases stress-related hormone secretion in women with hypothalamic amenorrhea
    Genazzani, AD
    Stomati, M
    Bersi, C
    Luisi, S
    Fedalti, M
    Santuz, M
    Esposito, G
    Petraglia, F
    Genazzani, AR
    [J]. JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, 2000, 23 (08) : 526 - 532
  • [6] Amino acid analysis in mammalian cell culture media containing serum and high glucose concentrations by anion exchange chromatography and integrated pulsed amperometric detection
    Genzel, Y
    König, S
    Reichl, U
    [J]. ANALYTICAL BIOCHEMISTRY, 2004, 335 (01) : 119 - 125
  • [7] EFFECTS OF DIURETICS ON GABA-GATED CHLORIDE CURRENT IN FROG ISOLATED SENSORY NEURONS
    INOMATA, N
    ISHIHARA, T
    AKAIKE, N
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1988, 93 (03) : 679 - 683
  • [8] Kim M. J., 2002, J KOREAN SOC FOOD SC, V31, P1071
  • [9] Physiological Characteristics and GABA Production of Lactobacillus acidophilus RMK567 Isolated from Raw Milk
    Lim, Sang-Dong
    Kim, Kee-Sung
    Do, Jeong-Ryong
    [J]. KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES, 2009, 29 (01) : 15 - 23
  • [10] Direct and Indirect Effects of Cannabinoids on in vitro GABA Release in the Rat Arcuate Nucleus
    Menzies, J. R. W.
    Ludwig, M.
    Leng, G.
    [J]. JOURNAL OF NEUROENDOCRINOLOGY, 2010, 22 (06) : 585 - 592
12