Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene-divinylbenzene beads

被引：102

作者：

Martins, Andrea B. ^{[1
]}

Friedrich, John L. R. ^{[1
]}

Cavalheiro, Jhonnattas C. ^{[1
]}

Garcia-Galan, Cristina ^{[3
]}

Barbosa, Oveimar ^{[3
,4
]}

Ayub, Marco A. Z. ^{[2
]}

Fernandez-Lafuente, Roberto ^{[3
]}

Rodrigues, Rafael C. ^{[1
]}

机构：

[1] Fed Univ Rio Grande Sul State, Inst Food Sci & Technol, Biocatalysis & Enzyme Technol Lab, BR-91501970 Porto Alegre, RS, Brazil

[2] Fed Univ Rio Grande Sul State, Inst Food Sci & Technol, Biochem Engn Lab, BiotecLab, BR-91501970 Porto Alegre, RS, Brazil

[3] ICP CSIC, Dept Biocatalysis, Madrid 28049, Spain

[4] Univ Ind Santander, Grp Invest Bioquim & Microbiol GIBIM, Escuela Quim, Bucaramanga, Colombia

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 134卷

关键词：

Interfacial activated lipases; Esterification; Butyl butyrate; TLL; Styrene-divinylbenzene beads; CANDIDA-ANTARCTICA; ESTER SYNTHESIS;

D O I：

10.1016/j.biortech.2013.02.052

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Two immobilized preparations from Thermomyces lanuginosus lipase (TLL) were compared in the synthesis of butyl butyrate. The commercial Lipozyme TL-IM, and TLL immobilized on styrene-divinylbenzene beads (MCI-TLL) were tested in the esterification reaction using n-hexane as solvent. The variables temperature (30-60 degrees C), substrate molar ratio (1:1 to 5:1), added water (0-1%), and biocatalyst content (3-40%) were evaluated in terms of initial reaction rate for each biocatalyst. SDS-PAGE analysis revealed that MCI-TLL had an immobilized enzymatic load twice as high as Lipozyme TL-IM, but with an activity 3-fold higher. MCI-TLL presented high initial reaction rates up to 1.0 M butyric acid, while Lipozyme TL-IM showed a decrease in its activity above 0.5 M. Moreover, MCI-TLL allowed a productivity of 14.5 mmol g(-1) h(-1), while Lipozyme TL-IM 3.2 mmol g(-1) h(-1), both by mass of biocatalyst. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：417 / 422

页数：6

共 18 条

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