Stability of collagen during denaturation

被引：23
|
作者：
Penkova, R
Goshev, I
Gorinstein, S
Nedkov, P
机构：
[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Sch Pharm, Dept Med Chem, IL-91120 Jerusalem, Israel
[2] Med Univ, Dept Biochem & Chem, Pleven 5800, Bulgaria
[3] Bulgarian Acad Sci, Inst Organ Chem, Ctr Phytochem, BU-1113 Sofia, Bulgaria
[4] David R Bloom Ctr Pharm, Jerusalem, Israel
来源：
JOURNAL OF PROTEIN CHEMISTRY | 1999年 / 18卷 / 04期
关键词：
collagen; glycerol; urea; sodium chloride; stabilization; denaturation;
D O I：
10.1023/A:1020632424142
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The stability of calf skin collagen (CSC) type I during thermal and chemical denaturation in the presence of glycerol was investigated. Thermal denaturation of type I collagen was performed in the presence of glycerol or in combination with urea and sodium chloride. The denaturation curves obtained in the presence of urea or sodium chloride retained their original shape without glycerol. These curves were shifted upward proportionally to the glycerol concentration in the reaction medium. This means that glycerol and the denaturants act independently. The explanation is based on the difference in the mechanism of their action on the collagen molecule.
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页码：397 / 401
页数：5
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