Denitrification in soil amended with thermophile-fermented compost suppresses nitrate accumulation in plants

被引:21
作者
Ishikawa, Kazuto [1 ]
Ohmori, Takashi [1 ]
Miyamoto, Hirokuni [2 ]
Ito, Toshiyuki [3 ]
Kumagai, Yoshifumi [3 ]
Sonoda, Masatoshi [1 ]
Matsumoto, Jirou [4 ]
Miyamoto, Hisashi [2 ]
Kodama, Hiroaki [5 ]
机构
[1] Chiba Univ, Grad Sch Hort, Chiba 2718501, Japan
[2] Miroku Co Ltd, Kitsuki City, Oita 8730000, Japan
[3] Keiyo Plant Engn Co Ltd, Ichikawa, Chiba 2720033, Japan
[4] Keiyo Gas Co Ltd, Ichikawa, Chiba 2720033, Japan
[5] Chiba Univ, Grad Sch Adv Integrat Sci, Chiba 2638522, Japan
基金
日本科学技术振兴机构;
关键词
Compost; Denitrification; Organic food; Plant nitrate concentration; Thermophile; DENITRIFYING BACTERIA; HUMIC SUBSTANCES; PIG MANURE; GENES; COMMUNITY; IDENTIFICATION; INHIBITION; EXPRESSION; GROWTH; NIA1;
D O I
10.1007/s00253-012-4004-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
NO (3) (-) is a major nitrogen source for plant nutrition, and plant cells store NO (3) (-) in their vacuoles. Here, we report that a unique compost made from marine animal resources by thermophiles represses NO (3) (-) accumulation in plants. A decrease in the leaf NO (3) (-) content occurred in parallel with a decrease in the soil NO (3) (-) level, and the degree of the soil NO (3) (-) decrease was proportional to the compost concentration in the soil. The compost-induced reduction of the soil NO (3) (-) level was blocked by incubation with chloramphenicol, indicating that the soil NO (3) (-) was reduced by chloramphenicol-sensitive microbes. The compost-induced denitrification activity was assessed by the acetylene block method. To eliminate denitrification by the soil bacterial habitants, soil was sterilized with gamma irradiation and then compost was amended. After the 24-h incubation, the N2O level in the compost soil with presence of acetylene was approximately fourfold higher than that in the compost soil with absence of acetylene. These results indicate that the low NO (3) (-) levels that are often found in the leaves of organic vegetables can be explained by compost-mediated denitrification in the soil.
引用
收藏
页码:1349 / 1359
页数:11
相关论文
共 37 条
[1]   Pig manure vermicompost as a component of a horticultural bedding plant medium: effects on physicochemical properties and plant growth [J].
Atiyeh, RM ;
Edwards, CA ;
Subler, S ;
Metzger, JD .
BIORESOURCE TECHNOLOGY, 2001, 78 (01) :11-20
[2]   INHIBITION OF EXISTING DENITRIFICATION ENZYME-ACTIVITY BY CHLORAMPHENICOL [J].
BROOKS, MH ;
SMITH, RL ;
MACALADY, DL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (05) :1746-1753
[3]   The need for reference materials when monitoring nitrate intake [J].
Castanheira, I ;
Oliveira, L ;
Valente, A ;
Alvito, P ;
Costa, HS ;
Alink, A .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2004, 378 (05) :1232-1238
[4]   Structure and activity of the denitrifying community in a maize-cropped field fertilized with composted pig manure or ammonium nitrate [J].
Dambreville, C ;
Hallet, S ;
Nguyen, C ;
Morvan, T ;
Germon, JC ;
Philippot, L .
FEMS MICROBIOLOGY ECOLOGY, 2006, 56 (01) :119-131
[5]   Ammonia oxidation, denitrification and dissimilatory nitrate reduction to ammonium in two US Great Basin hot springs with abundant ammonia-oxidizing archaea [J].
Dodsworth, Jeremy A. ;
Hungate, Bruce A. ;
Hedlund, Brian P. .
ENVIRONMENTAL MICROBIOLOGY, 2011, 13 (08) :2371-2386
[6]   Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization [J].
Enwall, K ;
Philippot, L ;
Hallin, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :8335-8343
[7]   Isolation, genetic and functional characterization of novel soil nirK-type denitrifiers [J].
Falk, Silke ;
Liu, Binbin ;
Braker, Gesche .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2010, 33 (06) :337-347
[8]   The effects of spacer sequences on silencing efficiency of plant RNAi vectors [J].
Hirai, Sayaka ;
Oka, Shin-ichiro ;
Adachi, Eri ;
Kodama, Hiroaki .
PLANT CELL REPORTS, 2007, 26 (05) :651-659
[9]   THE EFFECT OF OXYGEN ON DENITRIFICATION DURING STEADY-STATE GROWTH OF PARACOCCUS-HALODENITRIFICANS [J].
HOCHSTEIN, LI ;
BETLACH, M ;
KRITIKOS, G .
ARCHIVES OF MICROBIOLOGY, 1984, 137 (01) :74-78
[10]  
IPCC, 2007, Synthesis Report, DOI DOI 10.1017/CBO9780511546013