The expression of the embryonic gene Cripto-1 is regulated by OCT4 in human embryonal carcinoma NCCIT cells

被引：10

作者：

Park, Sung-Won ^{[1
]}

Do, Hyun-Jin ^{[1
]}

Han, Mi-Hee ^{[1
]}

Choi, Wonbin ^{[1
]}

Kim, Jae-Hwan ^{[1
]}

机构：

[1] CHA Univ, Coll Life Sci, Dept Biomed Sci, CHA Bio Complex,335 Pangyo Ro, Seongnam Si 13488, Gyeonggi Do, South Korea

来源：

FEBS LETTERS | 2018年 / 592卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Cripto-1; minimal promoter; NCCIT cells; OCT4; transcriptional activity; EPITHELIAL-MESENCHYMAL TRANSITION; STEM-CELLS; TRANSCRIPTION FACTOR; BREAST-CANCER; SELF-RENEWAL; PLURIPOTENCY; DIFFERENTIATION; TUMORIGENICITY; RESISTANCE; MARKER;

D O I：

10.1002/1873-3468.12935

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cripto-1 and OCT4, expressed in stem cells and cancers, play important roles in tumorigenesis. Here, we demonstrate that Cripto-1 expression is regulated by OCT4 in human embryonic carcinoma NCCIT cells. The endogenous expression of Cripto-1 and OCT4 is significantly reduced during differentiation. Cripto-1 expression is increased by OCT4 overexpression, but decreased by shRNA-mediated OCT4 knockdown. OCT4 overexpression significantly activates Cripto-1 transcriptional activity. A 5'-upstream minimal promoter sequence in the gene-encoding Cripto-1 is significantly activated by OCT4 overexpression. Mutation of the putative OCT4-binding site abolishes OCT4-mediated activation of the Cripto-1 promoter. The OCT4 transactivation domains mediate transcriptional activity of the Cripto-1 minimal promoter through direct interaction. Taken together, OCT4 plays an important role as a transcriptional activator of Cripto-1 expression in NCCIT cells.

引用

页码：24 / 35

页数：12

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