DNA-Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells

被引：47
|
作者：
Hsiao, Sonny C. [1 ,2 ]
Crow, Ailey K. [3 ]
Lam, Wilbur A. [4 ]
Bertozzi, Carolyn R. [2 ,5 ,6 ]
Fletcher, Daniel A. [3 ,4 ]
Francis, Matthew B. [1 ,2 ]
机构：
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] Lawrence Berkeley Lab, Div Mat Sci, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Biophys Grad Grp, Berkeley, CA 94720 USA
[4] Univ Calif Berkeley, UCSF Joint Grad Grp Bioengn, Berkeley, CA 94720 USA
[5] Univ Calif Berkeley, Dept Chem & Mol & Cell Biol, Berkeley, CA 94720 USA
[6] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 44期
关键词：
AFM; cell adhesion; DNA; metabolic engineering patterning;
D O I：
10.1002/anie.200802525
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Get a grip: Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells. (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：8473 / 8477
页数：5
相关论文
共 50 条
  • [31] BMP2 promotes migration and invasion of breast cancer cells via cytoskeletal reorganization and adhesion decrease: an AFM investigation
    Jin, Hua
    Pi, Jiang
    Huang, Xun
    Huang, Feicheng
    Shao, Wenxiang
    Li, Shengpu
    Chen, Yong
    Cai, Jiye
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 93 (04) : 1715 - 1723
  • [32] BMP2 promotes migration and invasion of breast cancer cells via cytoskeletal reorganization and adhesion decrease: an AFM investigation
    Hua Jin
    Jiang Pi
    Xun Huang
    Feicheng Huang
    Wenxiang Shao
    Shengpu Li
    Yong Chen
    Jiye Cai
    Applied Microbiology and Biotechnology, 2012, 93 : 1715 - 1723
  • [33] Live cell single-molecule imaging to study DNA repair in human cells
    Heyza, Joshua R.
    Mikhova, Mariia
    Schmidt, Jens C.
    DNA REPAIR, 2023, 129
  • [34] Investigation of Cell-Substrate Adhesion Properties of Living Chondrocyte by Measuring Adhesive Shear Force and Detachment Using AFM and Inverse FEA
    Trung Dung Nguyen
    YuanTong Gu
    Scientific Reports, 6
  • [35] Investigation of Cell-Substrate Adhesion Properties of Living Chondrocyte by Measuring Adhesive Shear Force and Detachment Using AFM and Inverse FEA
    Trung Dung Nguyen
    Yuan Tong Gu
    SCIENTIFIC REPORTS, 2016, 6
  • [36] AFM Characterization of Elastically Micropatterned Surfaces Fabricated by Fill-Molding In Capillaries (FIMIC) and Investigation of the Topographical Influence on Cell Adhesion to the Patterns
    Kelleher, Susan
    Jongerius, Aniek
    Loebus, Axel
    Strehmel, Christine
    Zhang, Zhenfang
    Lensen, Marga C.
    ADVANCED ENGINEERING MATERIALS, 2012, 14 (03) : B56 - B66
  • [37] Conjugation of DNA to streptavidin-coated quantum dots for the real-time imaging of gene transfer into live cells
    Kim, JK
    Lim, SH
    Lee, Y
    Shin, YS
    Chung, C
    Yoo, JY
    Chang, JK
    NSTI NANOTECH 2004, VOL 3, TECHNICAL PROCEEDINGS, 2004, : 379 - 382
  • [38] The effect of nano-coated titanium on cell adhesion and extracellular matrix formation in MG-63 Cells
    Seo, Sang-hui
    Kim, Yu-Ri
    Kim, In Kyoung
    Lee, Seung Ho
    Ryu, Jae-Jun
    Kim, Meyoung-Kon
    MOLECULAR & CELLULAR TOXICOLOGY, 2010, 6 (03) : 33 - 33
  • [39] Chronic Replication Problems Impact Cell Morphology and Adhesion of DNA Ligase I Defective Cells
    Cremaschi, Paolo
    Oliverio, Matteo
    Leva, Valentina
    Bione, Silvia
    Carriero, Roberta
    Mazzucco, Giulia
    Palamidessi, Andrea
    Scita, Giorgio
    Biamonti, Giuseppe
    Montecucco, Alessandra
    PLOS ONE, 2015, 10 (07):
  • [40] The anti-adhesive effect of glycoclusters on Pseudomonas aeruginosa bacteria adhesion to epithelial cells studied by AFM single cell force spectroscopy
    Zuttion, Francesca
    Ligeour, Caroline
    Vidal, Olivier
    Waelte, Mike
    Morvan, Francois
    Vidal, Sebastien
    Vasseur, Jean-Jacques
    Chevolot, Yann
    Phaner-Goutorbe, Magali
    Schillers, Hermann
    NANOSCALE, 2018, 10 (26) : 12771 - 12778
12345