DNA-Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells

被引：47

作者：

Hsiao, Sonny C. ^{[1
,2
]}

Crow, Ailey K. ^{[3
]}

Lam, Wilbur A. ^{[4
]}

Bertozzi, Carolyn R. ^{[2
,5
,6
]}

Fletcher, Daniel A. ^{[3
,4
]}

Francis, Matthew B. ^{[1
,2
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Lawrence Berkeley Lab, Div Mat Sci, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Biophys Grad Grp, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, UCSF Joint Grad Grp Bioengn, Berkeley, CA 94720 USA

[5] Univ Calif Berkeley, Dept Chem & Mol & Cell Biol, Berkeley, CA 94720 USA

[6] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 44期

关键词：

AFM; cell adhesion; DNA; metabolic engineering patterning;

D O I：

10.1002/anie.200802525

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Get a grip: Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells. (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8473 / 8477

页数：5

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