DNA-Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells

被引:47
|
作者
Hsiao, Sonny C. [1 ,2 ]
Crow, Ailey K. [3 ]
Lam, Wilbur A. [4 ]
Bertozzi, Carolyn R. [2 ,5 ,6 ]
Fletcher, Daniel A. [3 ,4 ]
Francis, Matthew B. [1 ,2 ]
机构
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] Lawrence Berkeley Lab, Div Mat Sci, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Biophys Grad Grp, Berkeley, CA 94720 USA
[4] Univ Calif Berkeley, UCSF Joint Grad Grp Bioengn, Berkeley, CA 94720 USA
[5] Univ Calif Berkeley, Dept Chem & Mol & Cell Biol, Berkeley, CA 94720 USA
[6] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
关键词
AFM; cell adhesion; DNA; metabolic engineering patterning;
D O I
10.1002/anie.200802525
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Get a grip: Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells. (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:8473 / 8477
页数:5
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