Protein Complexes and Target Genes Identification by in Vivo Biotinylation: The STAT5 Paradigm

被引：2
|
作者：
Katsantoni, Eleni [1 ]
机构：
[1] Acad Athens, Biomed Res Fdn, Div Hematol Oncol, Athens 11527, Greece
来源：
SCIENCE SIGNALING | 2012年 / 5卷 / 248期
关键词：
FUNCTIONAL INTERACTIONS; NUCLEAR TRANSLOCATION; TRANSCRIPTION FACTOR; ACTIVATION; PHOSPHORYLATION; EXPRESSION; INDUCTION; APOPTOSIS; RECEPTOR; BINDING;
D O I：
10.1126/scisignal.2003622
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Signal transducers and activators of transcription (STATs) are implicated in physiological cellular processes, stress-related responses, and malignancies. This presentation provides an overview on STATs, focusing on STAT5, and describes an in vivo biotinylation methodology applied in mammalian cells for identification of STAT5-containing protein complexes and STAT5 target genes. Our results show that in vivo biotinylation can be used efficiently to elucidate the complex STAT5-dependent transcriptional mechanisms.
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页数：4
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