Influence of the kainic acid on antioxidant status in the brain, liver and kidneys of the mouse

The effect of acute exposure of intraperitoneal injection of kainic acid (KA) on the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), amount of reduced glutathione (GSH) and content of ascorbic acid (ASC) has been studied in brain, liver and kidneys of male mice. Animals (n = 144) were treated KA with one dose, i.e. 12 mg/kg body weight or saline solution. The influence of KA on the tested parameters after 2, 4, 6 and 24 hours was estimated. After the injection of KA it has been found that the activity of SOD, GSHPx and CAT is very clearly reduced in the brain and to a lesser extent in liver and kidneys. Simultaneously a decrease in the activity of antioxidant enzymes in the brain, liver and kidneys was accompanied by a decrease of the amount of GSH and ASC. A decrease in the activity of SOD, CAT, GSHPx (after 2 and 4 h) and amount of GSH and ASC in the brain, liver and kidneys suggests that oxidative stress plays important role in the pathogenesis of KA in mice. The obtained results suggest that KA not only is toxic to the brain but also for the liver and kidneys of mice.