Separation of Variant Methylated Histone Tails by Differential Ion Mobility

被引：45
|
作者：
Shvartsburg, Alexandre A. [1 ]
Zheng, Yupeng [2 ]
Smith, Richard D. [1 ]
Kelleher, Neil L. [2 ]
机构：
[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA
[2] Northwestern Univ, Dept Chem, Dept Mol Biosci & Chem, Life Proc Inst, Evanston, IL 60208 USA
来源：
ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 15期
关键词：
DISSOCIATION MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; PEPTIDE ISOMERS; PHOSPHOPEPTIDES; LOCALIZATION; SITES;
D O I：
10.1021/ac301541r
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Differential ion mobility spectrometry (field asymmetric waveform ion mobility spectrometry (FAIMS)) is emerging as a broadly useful tool for separation of isomeric modified peptides with post-translational modifications (PTMs) attached to alternative residues. Such separations were anticipated to become more challenging for smaller PTMs and longer peptides. Here, we show that FAIMS can fully resolve localization variants involving a PTM as minuscule as methylation, even for larger peptides in the middle-down range.
引用
收藏
页码：6317 / 6320
页数：4
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