Detection of doxorubicin-induced apoptosis of leukemic T-lymphocytes by laser tweezers Raman spectroscopy

被引:52
作者
Moritz, Tobias J. [1 ,2 ]
Taylor, Douglas S. [1 ,3 ]
Krol, Denise M. [2 ,4 ]
Fritch, John [1 ]
Chan, James W. [1 ]
机构
[1] Univ Calif Davis, NSF Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA
[2] Univ Calif Davis, Biophys Grad Grp, Davis, CA 95616 USA
[3] Univ Calif Davis, Med Ctr, Dept Pediat, Sacramento, CA 95817 USA
[4] Univ Calif Davis, Dept Appl Sci, Davis, CA 95616 USA
基金
美国国家科学基金会;
关键词
D O I
10.1364/BOE.1.001138
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Laser tweezers Raman spectroscopy (LTRS) was used to acquire the Raman spectra of leukemic T lymphocytes exposed to the chemotherapy drug doxorubicin at different time points over 72 hours. Changes observed in the Raman spectra were dependent on drug exposure time and concentration. The sequence of spectral changes includes an intensity increase in lipid Raman peaks, followed by an intensity increase in DNA Raman peaks, and finally changes in DNA and protein (phenylalanine) Raman vibrations. These Raman signatures are consistent with vesicle formation, cell membrane blebbing, chromatin condensation, and the cytoplasm of dead cells during the different stages of drug-induced apoptosis. These results suggest the potential of LTRS as a real-time single cell tool for monitoring apoptosis, evaluating the efficacy of chemotherapeutic treatments, or pharmaceutical testing. (C)2010 Optical Society of America
引用
收藏
页码:1138 / 1147
页数:10
相关论文
共 45 条
[1]   Monitoring of minimal residual disease in acute myeloid leukemia [J].
Buccisano, Francesco ;
Maurillo, Luca ;
Spagnoli, Alessandra ;
Del Principe, Maria Ilaria ;
Ceresoli, Eleonora ;
Lo Coco, Francesco ;
Arcese, William ;
Amadori, Sergio ;
Venditti, Adriano .
CURRENT OPINION IN ONCOLOGY, 2009, 21 (06) :582-588
[2]   Detection of drug-induced cellular changes using confocal Raman spectroscopy on patterned single-cell biosensors [J].
Buckmaster, Ryan ;
Asphahani, Fareid ;
Thein, Myo ;
Xu, Jian ;
Zhang, Miqin .
ANALYST, 2009, 134 (07) :1440-1446
[3]   Raman spectroscopy and microscopy of individual cells and cellular components [J].
Chan, James ;
Fore, Samantha ;
Wachsman-Hogiu, Sebastian ;
Huser, Thomas .
LASER & PHOTONICS REVIEWS, 2008, 2 (05) :325-349
[4]   Nondestructive identification of individual leukemia cells by laser trapping Raman spectroscopy [J].
Chan, James W. ;
Taylor, Douglas S. ;
Lane, Stephen M. ;
Zwerdling, Theodore ;
Tuscano, Joseph ;
Huser, Thomas .
ANALYTICAL CHEMISTRY, 2008, 80 (06) :2180-2187
[5]   Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells [J].
Chan, JW ;
Taylor, DS ;
Zwerdling, T ;
Lane, SM ;
Ihara, K ;
Huser, T .
BIOPHYSICAL JOURNAL, 2006, 90 (02) :648-656
[6]   Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy [J].
Chan, JW ;
Esposito, AP ;
Talley, CE ;
Hollars, CW ;
Lane, SM ;
Huser, T .
ANALYTICAL CHEMISTRY, 2004, 76 (03) :599-603
[7]   Treatment-specific changes in gene expression discriminate in vivo drug response in human leukemia cells [J].
Cheok, MH ;
Yang, WL ;
Pui, CH ;
Downing, JR ;
Cheng, C ;
Naeve, CW ;
Relling, MV ;
Evans, WE .
NATURE GENETICS, 2003, 34 (01) :85-90
[8]   Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I [J].
Coleman, ML ;
Sahai, EA ;
Yeo, M ;
Bosch, M ;
Dewar, A ;
Olson, MF .
NATURE CELL BIOLOGY, 2001, 3 (04) :339-345
[9]   Apoptosis and cancer: the genesis of a research field [J].
Cotter, Thomas G. .
NATURE REVIEWS CANCER, 2009, 9 (07) :501-507
[10]   Comparison of anthracycline-induced death of human leukemia cells: Programmed cell death versus necrosis [J].
Dartsch, DC ;
Schaefer, A ;
Boldt, S ;
Kolch, W ;
Marquardt, H .
APOPTOSIS, 2002, 7 (06) :537-548