Histopathological and biochemical effects of cyanobacterial cells containing microcystin-LR on Tilapia fish

被引：3

作者：

Preeti, T. ^{[1
]}

Hariharan, G. ^{[2
]}

Rajarajeswari, G. R. ^{[1
]}

机构：

[1] Anna Univ, Dept Chem, Coll Engn Guindy, Madras 600025, Tamil Nadu, India

[2] Anna Univ, Inst Ocean Management, Madras 600025, Tamil Nadu, India

来源：

WATER AND ENVIRONMENT JOURNAL | 2016年 / 30卷 / 1-2期

关键词：

biochemical alteration; cyanobacteria; histopathology; Microcystis aeruginosa; microcystin-LR; Oreochromis niloticus; Thenneri lake; toxicity; OREOCHROMIS SP; GLUTATHIONE; ACID; PURIFICATION; HEPATOCYTES; TOXICITY; STRAINS; TOXIN; LIVER;

D O I：

10.1111/wej.12169

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Blooms of Microcystis aeruginosa, commonly observed in many lakes of Tamil Nadu, produce the cyanobacterial toxin Microcystin, which has tumor promoting property in fish. Strains of Microcystis aeruginosa species have the same morphology but may vary in their toxicity. In the present study, Microcystin produced by Microcystis aeruginosa from Thenneri lake in Kancheepuram district of Tamil Nadu, was isolated and characterized by high-performance liquid chromatography and electron spray ionization mass spectroscopy. Based on spectroscopic results the toxin was identified as Microcystin-LR. Toxicogenicity was evident from the alteration in the activity of antioxidant enzymes [Glutathione S-transferase and Catalase] and increase of protein and lipid peroxidation contents in Oreochromis niloticus, a fresh water fish species, after controlled exposure to Microcystis aeruginosa cells. Toxicity was further confirmed by histopathological changes in gill, liver and intestine of exposed fish.

引用

页码：135 / 142

页数：8

共 33 条

[21] Responses of antioxidant systems in the hepatocytes of common carp (Cyprinus carpio L.) to the toxicity of microcystin-LR [J].

Li, XY ;

Liu, YD ;

Song, LR ;

Liu, HT .

TOXICON, 2003, 42 (01) :85-89

[22]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[23] Acid and alkaline phosphatase activities and pathological changes induced in Tilapia fish (Oreochromis sp.) exposed subchronically to microcystins from toxic cyanobacterial blooms under laboratory conditions [J].

Molina, R ;

Moreno, I ;

Pichardo, S ;

Jos, A ;

Moyano, R ;

Monterde, JG ;

Cameán, A .

TOXICON, 2005, 46 (07) :725-735

[24] ASSAY FOR LIPID PEROXIDES IN ANIMAL-TISSUES BY THIOBARBITURIC ACID REACTION [J].

OHKAWA, H ;

OHISHI, N ;

YAGI, K .

ANALYTICAL BIOCHEMISTRY, 1979, 95 (02) :351-358

[25] Toxicological evaluation of microcystins in aquatic fish species: Current knowledge and future directions [J].

Pavagadhi, Shruti ;

Balasubramanian, Rajasekhar .

AQUATIC TOXICOLOGY, 2013, 142 :1-16

[26] Optimization of laboratory scale production and purification of microcystin-LR from pure cultures of Microcystis aeruginosa [J].

Phelan, R. R. ;

Downing, T. G. .

AFRICAN JOURNAL OF BIOTECHNOLOGY, 2007, 6 (21) :2451-2457

[27] Isolation and characterization of microcystin producing Microcystis from a Central Indian water bloom [J].

Sangolkar, Lalita N. ;

Maske, Sarika S. ;

Muthal, Pradeep L. ;

Kashyap, Sanjay M. ;

Chakrabarti, Tapan .

HARMFUL ALGAE, 2009, 8 (05) :674-684

[28]

Sengpracha Waya, 2006, Chiang Mai Journal of Science, V33, P231

[29] ISOLATION AND CHARACTERIZATION OF A VARIETY OF MICROCYSTINS FROM 7 STRAINS OF THE CYANOBACTERIAL GENUS ANABAENA [J].

SIVONEN, K ;

NAMIKOSHI, M ;

EVANS, WR ;

CARMICHAEL, WW ;

SUN, F ;

ROUHIAINEN, L ;

LUUKKAINEN, R ;

RINEHART, KL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (08) :2495-2500

[30]

Smith GilbertM., 1950, FRESH WATER ALGAE US, P1

← 1 2 3 4 →