Molecular epidemiology of PRRSV in South China from 2007 to 2011 based on the genetic analysis of ORF5

被引：33

作者：

Xie, Jiexiong ^{[1
]}

Zhu, Wanjun ^{[1
]}

Chen, Ye ^{[1
]}

Wei, Chunya ^{[1
]}

Zhou, Pei ^{[1
]}

Zhang, Minze ^{[1
]}

Huang, Zhen ^{[1
]}

Sun, Long ^{[1
]}

Su, Shuo ^{[1
]}

Zhang, Guihong ^{[1
]}

机构：

[1] South China Agr Univ, Coll Vet Med, Minist China, MOA Key Lab Anim Vaccine Dev, Guangzhou 510642, Guangdong, Peoples R China

来源：

MICROBIAL PATHOGENESIS | 2013年 / 63卷

基金：

中国国家自然科学基金;

关键词：

Porcine reproductive and respiratory syndrome virus (PRRSV); GP5; Genetic diversity; South China; RESPIRATORY SYNDROME VIRUS; LELYSTAD-VIRUS; NORTH-AMERICAN; DIVERSITY; SEQUENCE; MUTATIONS; EMERGENCE; PROTEINS; VACCINE; GENOME;

D O I：

10.1016/j.micpath.2013.05.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Porcine reproductive and respiratory syndrome virus (PRRSV) has proven to be highly genetically variable; however, comprehensive information regarding the virus's genetic diversity in South China is limited. In this study, a total of 3199 clinical samples were collected from 267 pig farms suspected of PRRSV infection between 2007 and 2011. The ORF5 genes of 51 PRRSV-positive samples were sequenced and analyzed. The 51 study strains were divided into three primary subgenotypes. Fourty-five of the strains belonged to subgenotype I and were closely related to the highly pathogenic PRRSV (HP-PRRSV) strains. The subgenotype I strains were generally clustered into genetically similar groups by year. Only one of the strains belonged to subgenotype II, clustering with the classical North American type, VR2332. Five of the strains were grouped into subgenotype III, which occupied a separate branch and was closely related to the recently isolated novel field strains, QYYZ and GM2. The 5 subgenotype III strains shared an amino acid identity with the remaining 46 study strains ranging from 79.6%-83.6%. Amino acid analysis showed extensive mutations in subgenotype III; the diverse genetic mutations of these novel strains are of great concern. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：30 / 36

页数：7

共 33 条

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