Quantitative detection and proliferation dynamics of a novel Spiroplasma eriocheiris pathogen in the freshwater crayfish, Procambarus clarkii

被引：32

作者：

Ding, Zhengfeng ^{[1
,2
,3
]}

Tang, Jianqing ^{[3
]}

Xue, Hui ^{[3
]}

Li, Jiajia ^{[3
]}

Ren, Qian ^{[1
,2
]}

Gu, Wei ^{[1
,2
]}

Meng, Qingguo ^{[1
,2
]}

Wang, Wen ^{[1
,2
]}

机构：

[1] Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing 210046, Jiangsu, Peoples R China

[2] Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Aquat Crustacean Dis, Nanjing 210046, Jiangsu, Peoples R China

[3] Freshwater Fisheries Res Inst Jiangsu Prov, Nanjing 210017, Jiangsu, Peoples R China

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2014年 / 115卷

关键词：

Spiroplasma eriocheiris; Real-time PCR; SYBR Green; Quantitation; REAL-TIME PCR; PENAEUS-VANNAMEI; NECROSIS VIRUS; SHRIMP; CHINA; QUANTIFICATION; CRUSTACEANS; INFECTION; SINENSIS; DISEASE;

D O I：

10.1016/j.jip.2013.10.012

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Spiroplasma eriocheiris disease control based on sensitive quantitative methods has become a priority. A SYBR Green real-time PCR that can simultaneously detect and quantify S. eriocheiris in the freshwater crayfish Procambarus clarkii was produced and evaluated. In the asymptomatic crayfish, hemolymph exhibited the statistically greatest number of S. eriocheiris copies indicating a tissue-specific pathogen infection characteristic. The curve of the pathogen amount change in vivo assumed a very similar shape with the typical one-step growth curve. A turning point from chronic infection to acute infection was suggested from 3 to 4 days when the S. eriocheiris copies in hemolymph increased substantially. (C) 2013 Published by Elsevier Inc.

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收藏

页码：51 / 54

页数：4

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