Size effect of hydroxyapatite nanoparticles on proliferation and apoptosis of osteoblast-like cells

被引：397

作者：

Shi, Zhongli ^{[1
,2
]}

Huang, Xin ^{[1
]}

Cai, Yurong ^{[3
,4
,5
]}

Tang, Ruikang ^{[3
,4
]}

Yang, Disheng ^{[1
]}

机构：

[1] Med Coll Zhejiang Univ, Dept Orthopaed Surg, Affiliated Hosp 2, Hangzhou 310009, Zhejiang, Peoples R China

[2] Zhejiang Univ, Inst Orthopaed Res, Hangzhou 310027, Zhejiang, Peoples R China

[3] Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China

[4] Zhejiang Univ, Ctr Biopathways & Biomat, Hangzhou 310027, Zhejiang, Peoples R China

[5] Zhejiang Sci Tech Univ, Coll Mat & Text, Key Lab Adv Text Mat & Mfg Technol, Minist Educ, Hangzhou 310018, Zhejiang, Peoples R China

来源：

ACTA BIOMATERIALIA | 2009年 / 5卷 / 01期

关键词：

Size effect; Nano-hydroxyapatite; Osteoblast-like cells; Cell proliferation; Cell apoptosis; NANOSTRUCTURED HYDROXYAPATITE; CALCIUM-PHOSPHATE; BONE; ADHESION; BIOCOMPATIBILITY; CRYSTALLINITY; MECHANISM; CERAMICS; HA;

D O I：

10.1016/j.actbio.2008.07.023

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Nano-hydroxyapatite (nano-HAP) may be a better candidate for an apatite substitute of bone in biomedical applications than microsized hydroxyapatite (m-HAP). However, size control is always difficult when synthesizing well-defined nano-HAP particles. In this study, nano-HAP particles with diameters of similar to 20 nm (np20) and similar to 80 nm (np80) were synthesized and characterized. The size effects of these nano-HAPs and m-HAP were studied on human osteoblast-like MG-63 cells in vitro. Our results demonstrate that both cell proliferation and cell apoptosis are related to the size of the HAP particles. Np20 has the best effect on promotion of cell growth and inhibition of cell apoptosis. This work provides an interesting view of the role of nano-HAPs as ideal biomedical materials in future clinical applications. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：338 / 345

页数：8

共 35 条

[1] Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD [J].