Efficient isolation method for human recombinant tumor necrosis factor alpha

被引：0
|
作者：
Tikhonov, RV [1 ]
Yakimov, SA [1 ]
Korobko, VG [1 ]
Wulfson, AN [1 ]
机构：
[1] RUSSIAN ACAD SCI, MM SHEMYAKIN YA OVCHINNIKOV INST BIOORGAN CHEM, MOSCOW 117871, RUSSIA
来源：
BIOORGANICHESKAYA KHIMIYA | 1996年 / 22卷 / 03期
关键词：
human recombinant tumor necrosis factor alpha; chromatography;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An efficient and productive isolation method for human recombinant tumor necrosis factor a from Escherichia coli cells was developed. The method includes a membrane filtration step, two steps of ion-exchange chromatography, and gel filtration on a Sephadex G-25 column. The target product was obtained with approximately 50% total yield and greater than 95% purity according to PAGE and HPLC.
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页码：163 / 167
页数：5
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