The effect of red blood cells on thrombin generation

被引：99

作者：

Horne, MK ^{[1
]}

Cullinane, AM ^{[1
]}

Merryman, PK ^{[1
]}

Hoddeson, EK ^{[1
]}

机构：

[1] NIH, Hematol Serv, Dept Lab Med, Warren G Magnuson Clin Ctr, Bethesda, MD 20892 USA

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2006年 / 133卷 / 04期

关键词：

thrombin; red cells; platelets; thrombophilia; blood coagulation;

D O I：

10.1111/j.1365-2141.2006.06047.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have developed an assay of thrombin generation in clotting whole blood. Because our goal was to study patients with red blood cell diseases that are associated with thrombosis, the initial evaluation of this assay analysed the effect of the haematocrit and haemolysate on the total amount of thrombin activity generated and the maximal concentration of thrombin achieved. Both of these parameters were proportional to the haematocrit throughout a wide range of clinically relevant cell concentrations. Red cell lysate also augmented thrombin generation. Because this effect was removed by filtration of the lysate, we propose that it was related to red cell microparticles.

引用

页码：403 / 408

页数：6

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