Speciation of seleno compounds in yeast aqueous extracts by three-dimensional liquid chromatography with inductively coupled plasma mass spectrometric and electrospray mass spectrometric detection

被引：81
作者：
McSheehy, S [1 ]
Pannier, F [1 ]
Szpunar, J [1 ]
Potin-Gautier, M [1 ]
Lobinski, R [1 ]
机构：
[1] Univ Pau & Pays Adour, Grp Bioinorgan Analyt Chem, CNRS, UMR 5034, F-64053 Pau, France
来源：
ANALYST | 2002年 / 127卷 / 02期
关键词：
D O I：
10.1039/b108680b
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A three-dimensional liquid chromatographic purification protocol based on sequential size-exclusion, anion-exchange and cation-exchange separation mechanisms was developed for the mapping of seleno compounds in aqueous yeast extracts. The method allowed the demonstration of the presence of more than 30 different seleno compounds. Semi-preparative size-exclusion and anion-exchange chromatography were optimized for maximum resolution using electrospray-compatible buffers in order to purify the compounds for mass spectrometric analysis. Molecular masses were attributed to many of the compounds on the basis of the selenium isotopic pattern in the electrospray mass spectra and of the collision-induced fragmentation patterns. Limitations preventing the ultimate identification of the selenium species detected are discussed.
引用
收藏
页码：223 / 229
页数：7
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