Identification of proteins specifically interacting with YB-1 mRNA 3′ UTR and the effect of hnRNP Q on YB-1 mRNA translation

被引：16

作者：

Lyabin, D. N. ^{[1
]}

Nigmatullina, L. F. ^{[1
]}

Doronin, A. N. ^{[1
]}

Eliseeva, I. A. ^{[1
]}

Ovchinnikov, L. P. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2013年 / 78卷 / 06期

基金：

俄罗斯基础研究基金会;

关键词：

protein YB-1; protein hnRNP Q; translation regulation; 3 ' untranslated region of mRNA; TRANSCRIPTION FACTOR YB-1; BOX-BINDING-PROTEIN; POLY(A)-BINDING PROTEIN; NUCLEAR TRANSLOCATION; DEPENDENT TRANSLATION; IN-VITRO; EXPRESSION; DNA; REPAIR; LOCALIZATION;

D O I：

10.1134/S0006297913060102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, proteins specifically interacting with the 3' untranslated region (UTR) of mRNA of the multifunctional Y-box-binding protein 1 (YB-1) were identified. One of these, hnRNP Q, was shown to specifically interact with the regulatory element (RE) in YB-1 mRNA 3' UTR and to inhibit translation of this mRNA. Its binding to the RE was accompanied by displacement from this element of the poly(A)-binding protein (PABP), a positive regulator of YB-1 mRNA translation, and by enhanced binding of the negative YB-1 mRNA translation regulator - YB-1 itself.

引用

页码：651 / 659

页数：9

共 45 条

[1] Nuclear localization and increased levels of transcription factor YB-1 in primary human breast cancers are associated with intrinsic MDR1 gene expression [J].

Bargou, RC ;

Jurchott, K ;

Wagener, C ;

Bergmann, S ;

Metzner, S ;

Bommert, K ;

Mapara, MY ;

Winzer, KJ ;

Dietel, M ;

Dorken, B ;

Royer, HD .

NATURE MEDICINE, 1997, 3 (04) :447-450

[2] UNR, a new partner of poly(A)-binding protein, plays a key role in translationally coupled mRNA turnover mediated by thr c-fos major coding-region determinant [J].

Chang, TC ;

Yamashita, A ;

Chen, CYA ;

Yamashita, Y ;

Zhu, WM ;

Durdan, S ;

Kahvejian, A ;

Sonenberg, N ;

Shyu, AB .

GENES & DEVELOPMENT, 2004, 18 (16) :2010-2023

[3]

Chansky HA, 2001, CANCER RES, V61, P3586

[4] Minor secondary-structure variation in the 5′-untranslated region of the β-globin mRNA changes the concentration requirements for eIF2 [J].

Dmitriev, SE ;

Terenin, IM ;

Rubtsova, MP ;

Shatsky, IN .

MOLECULAR BIOLOGY, 2003, 37 (03) :421-428

[5] Y-box-binding protein 1 (YB-1) and its functions [J].

Eliseeva, I. A. ;

Kim, E. R. ;

Guryanov, S. G. ;

Ovchinnikov, L. P. ;

Lyabin, D. N. .

BIOCHEMISTRY-MOSCOW, 2011, 76 (13) :1402-1433

[6] Specific PABP effect on translation of YB-1 mRNA is neutralized by polyadenylation through a "mini-loop" at 3′ UTR [J].

Eliseeva, Irina A. ;

Ovchinnikov, Lev P. ;

Lyabin, Dmitry N. .

RNA BIOLOGY, 2012, 9 (12) :1473-1487

[7] The major mRNA-associated protein YBA is a potent 5′ cap-dependent mRNA stabilizer [J].

Evdokimova, V ;

Ruzanov, P ;

Imataka, H ;

Raught, B ;

Svitkin, Y ;

Ovchinnikov, LP ;

Sonenberg, N .

EMBO JOURNAL, 2001, 20 (19) :5491-5502

[8] Akt-mediated YB-1 phosphorylation activates translation of silent mRNA species [J].

Evdokimova, V ;

Ruzanov, P ;

Anglesio, MS ;

Sorokin, AV ;

Ovchinnikov, LP ;

Buckley, J ;

Triche, TJ ;

Sonenberg, N ;

Sorensen, PHB .

MOLECULAR AND CELLULAR BIOLOGY, 2006, 26 (01) :277-292

[9] Y-box protein-1 is actively secreted through a non-classical pathway and acts as an extracellular mitogen [J].

Frye, Bjoern C. ;

Halfter, Sarah ;

Djudjaj, Sonja ;

Muehlenberg, Philipp ;

Weber, Susanne ;

Raffetseder, Ute ;

En-Nia, Abdelaziz ;

Knott, Hanna ;

Baron, Jens M. ;

Dooley, Steven ;

Bernhagen, Juergen ;

Mertens, Peter R. .

EMBO REPORTS, 2009, 10 (07) :783-789

[10] YB-1 promotes strand separation in vitro of duplex DNA containing either mispaired bases or cisplatin modifications, exhibits endonucleolytic activities and binds several DNA repair proteins [J].

Gaudreault, I ;

Guay, D ;

Lebel, M .

NUCLEIC ACIDS RESEARCH, 2004, 32 (01) :316-327

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