Hordenine: A Novel Quorum Sensing Inhibitor and Antibiofilm Agent against Pseudomonas aeruginosa

被引：166
作者：
Zhou, Jin-Wei [1 ,2 ]
Luo, Huai-Zhi [1 ,2 ]
Jiang, Huan [1 ,2 ]
Jian, Ting-Kun [1 ,2 ]
Chen, Zi-Qian [1 ,2 ]
Jia, Ai-Qun [1 ]
机构：
[1] Hainan Univ, Key Lab Trop Biol Resources, State Key Lab Marine Resource Utilizat South Chin, Minist Educ, Haikou 570228, Hainan, Peoples R China
[2] Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 07期
基金：
中国国家自然科学基金;
关键词：
hordenine; foodborne pathogen; Pseudomonas aeruginosa; quorum sensing; biofilm; FOODBORNE PATHOGENS; CYSTIC-FIBROSIS; ANTI-BIOFILM; IN-VITRO; SUSCEPTIBILITY; METABOLITES; DERIVATIVES; RESISTANCE; BACTERIA; INCREASE;
D O I：
10.1021/acs.jafc.7b05035
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The quorum sensing (QS) inhibitory activity of hordenine from sprouting barley against foodborne pathogen Pseudomonas aeruginosa was evaluated for the first time here. At concentrations ranging from 0.5 to 1.0 mg mL(-1), hordenine inhibited the levels of acyl-homoserine lactones. The enhanced susceptibility of hordenine with netilmicin on P. aeruginosa PAO1 biofilm formation as well as their efficiency in disrupting preformed biofilms was also evaluated using scanning electron microscopy and confocal laser scanning microscopy (CLSM). Hordenine treatment inhibited the :production of QS-related extracellular virulence factors of P. aeruginosa PAO1. Additionally, quantitative real-time polymerase chain reaction analysis demonstrated that the expressions of QS-related genes, IasI, lasR, rhlI, and rhlR, were significantly suppressed. Our results indicated that hordenine can serve as a competitive inhibitor for signaling molecules and act as a novel QS-based agent to defend against foodborne pathogens.
引用
收藏
页码：1620 / 1628
页数：9
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