Ethanol production from henequen (Agave fourcroydes Lem.) juice and molasses by a mixture of two yeasts

被引:28
作者
Caceres-Farfan, Mirbella [1 ]
Lappe, Patricia [2 ]
Larque-Saavedra, Alfonso [1 ]
Magdub-Mendez, Abdo [1 ]
Barahona-Perez, Luis [1 ]
机构
[1] Ctr Invest Cient Yucatan, Merida 97200, Yucatan, Mexico
[2] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico
关键词
Henequen; Alcohol; Kluyveromyces marxianus; Saccharomyces cerevisiae; Sugars;
D O I
10.1016/j.biortech.2008.04.063
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
In the fermentation process of henequen (Agave fourcroydes Lem.) leaf juice, complemented with industrial molasses, the use of an inoculum comprising two yeasts: Kluyveromyces marxianus (isolated from the henequen plant) and Saccharomyces cerevisiae (commercial strain) was studied. An ethanol production of 5.22 +/- 1.087% v/v was obtained. Contrary to expected, a decrease on ethanol production was observed with the use of the K. marxianus strain. The best results were obtained when a mixture of 25% K. marxianus and 75% S. cerevisiae or S. cerevisiae alone were used with an initial inoculum concentration of 3 x 10(7) Cell mL(-1). Furthermore, it was possible to detect a final concentration of approximately 2 - 4 g L-1 of reducing sugars that are not metabolized by the yeasts for the ethanol production. These results show that although the use of a mixture of yeasts can be of interest for the production of alcoholic beverages, it can be the opposite in the case of ethanol production for industrial purposes where manipulation of two strains can raise the production costs (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:9036 / 9039
页数:4
相关论文
共 18 条
  • [1] FERMENTATION OF STARCH TO ETHANOL BY A COMPLEMENTARY MIXTURE OF AN AMYLOLYTIC YEAST AND SACCHAROMYCES-CEREVISIAE
    ABOUZIED, MM
    REDDY, CA
    [J]. BIOTECHNOLOGY LETTERS, 1987, 9 (01) : 59 - 62
  • [2] BARBOURGENIN, A NEW STEROIDAL SAPOGENIN FROM AGAVE-SISALANA LEAVES
    BLUNDEN, G
    PATEL, AV
    CRABB, TA
    [J]. JOURNAL OF NATURAL PRODUCTS, 1986, 49 (04): : 687 - 689
  • [3] CEDENOCRUZ M, 1995, CRIT REV BIOTECHNOL, V5, P11
  • [4] Molecular characterization of Kluyveromyces marxianus strains isolated from agave fourcroydes (Lem.) in yucatan, Mexico
    Daisy, Perez-Brito
    Raul, Tapia-Tussell
    Quijano-Ramayo, Andres
    Larque-Saavedra, Alfonso
    Lappe, Patricia
    [J]. MOLECULAR BIOTECHNOLOGY, 2007, 37 (03) : 181 - 186
  • [5] DING Y, 1989, PHYTOCHEMISTRY, V28, P2787, DOI 10.1016/S0031-9422(00)98089-0
  • [6] Yeast interactions and wine flavour
    Fleet, GH
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 86 (1-2) : 11 - 22
  • [7] GARICAALBORNOZ MA, 2006, THESIS CTR INVESTIGA
  • [8] Fermentation of molasses using a thermotolerant yeast, Kluyveromyces marxianus IMB3: Simplex optimisation of media supplements
    Gough, S
    Flynn, O
    Hack, CJ
    Marchant, R
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 46 (02) : 187 - 190
  • [9] Bio-ethanol -: the fuel of tomorrow from the residues of today
    Hahn-Hagerdal, B.
    Galbe, M.
    Gorwa-Grauslund, M. F.
    Liden, G.
    Zacchi, G.
    [J]. TRENDS IN BIOTECHNOLOGY, 2006, 24 (12) : 549 - 556
  • [10] Utilization of corn silage juice by Klyuveromyces marxianus
    Hang, YD
    Woodams, EE
    Hang, LE
    [J]. BIORESOURCE TECHNOLOGY, 2003, 86 (03) : 305 - 307