A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus

被引：27

作者：

Fan, Qing ^{[1
,2
]}

Xie, Zhixun ^{[1
,2
]}

Xie, Liji ^{[1
,2
]}

Liu, Jiabo ^{[1
,2
]}

Pang, Yaoshan ^{[1
,2
]}

Deng, Xianwen ^{[1
,2
]}

Xie, Zhiqin ^{[1
,2
]}

Peng, Yi ^{[1
,2
]}

Wang, Xiuqing ^{[3
]}

机构：

[1] Guangxi Vet Res Inst, Dept Biotechnol, Nanning 530001, Guangxi, Peoples R China

[2] Guangxi Key Lab Anim Vaccines & Diagnost, Nanning 530001, Guangxi, Peoples R China

[3] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2012年 / 186卷 / 1-2期

关键词：

Bovine viral diarrhea virus (BVDV); 5 ' untranslated region (5 ' UTR); Reverse transcription loop-mediated isothermal amplification (RT-LAMP); INFECTION; DIAGNOSIS; SAMPLES; ASSAY; DNA;

D O I：

10.1016/j.jviromet.2012.08.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RI-LAMP assay is highly sensitive and able to detect 4.67 x 10(0) copies of BVDV RNA. Additionally, the RI-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：43 / 48

页数：6

共 25 条

[1] Development of reverse transcription loop-mediated isothermal amplification for rapid detection of H9 avian influenza virus [J].

Chen, Hao-tai ;

Zhang, Jie ;

Sun, De-hui ;

Ma, Li-na ;

Liu, Xiang-tao ;

Cai, Xue-peng ;

Liu, Yong-sheng .

JOURNAL OF VIROLOGICAL METHODS, 2008, 151 (02) :200-203

[2]

Chen Lei, 2010, Virologica Sinica, V25, P59, DOI 10.1007/s12250-010-3043-2

[3]

Cho HS, 2006, J VET DIAGN INVEST, V18, P81

[4] A comparison of polymerase chain reaction with and without RNA extraction and virus isolation for detection of bovine viral diarrhea virus in young calves [J].

Deregt, D ;

Carman, PS ;

Clark, RM ;

Burton, KM ;

Olson, WO ;

Gilbert, SA .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2002, 14 (05) :433-437

[5] Rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method [J].

Enomoto, Y ;

Yoshikawa, T ;

Ihira, M ;

Akimoto, S ;

Miyake, F ;

Usui, C ;

Suga, S ;

Suzuki, K ;

Kawana, T ;

Nishiyama, Y ;

Asano, Y .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (02) :951-955

[6]

Fan Q., 2010, PROGR VET MED, V31, P10

[7] Evaluation of diagnostic tests used for detection of bovine viral diarrhea virus and prevalence of subtypes 1a, 1b, and 2a in persistently infected cattle entering a feedlot [J].

Fulton, RW ;

Hessman, B ;

Johnson, BJ ;

Ridpath, JF ;

Saliki, JT ;

Burge, LJ ;

Sjeklocha, D ;

Confer, AW ;

Funk, RA .

JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 2006, 228 (04) :578-584

[8] Simple procedures to obtain exogenous internal controls for use in RT-PCR detection of bovine pestiviruses [J].

Golemba, Marcelo D. ;

Parreno, Viviana ;

Jones, Leandro R. .

MOLECULAR AND CELLULAR PROBES, 2008, 22 (03) :212-214

[9] RAPID DETECTION OF BOVINE VIRAL DIARRHEA VIRUS BY USING RNA EXTRACTED DIRECTLY FROM ASSORTED SPECIMENS AND A ONE-TUBE REVERSE TRANSCRIPTION PCR ASSAY [J].

HAMEL, AL ;

WASYLYSHEN, MD ;

NAYAR, GPS .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (02) :287-291

[10] EPIDEMIOLOGY OF BOVINE VIRAL DIARRHEA VIRUS [J].

HOUE, H .

VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 1995, 11 (03) :521-&

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