Conjugation and Evaluation of Triazole-Linked Single Guide RNA for CRISPR-Cas9 Gene Editing

被引：25

作者：

He, Kaizhang ^{[1
]}

Chou, Eldon T. ^{[1
]}

Begay, Shawn ^{[1
]}

Anderson, Emily M. ^{[1
]}

Smith, Anja van Brabant ^{[1
]}

机构：

[1] GE Healthcare Dharmacon Inc, 2650 Crescent Dr,Suite 100, Lafayette, CO 80026 USA

来源：

CHEMBIOCHEM | 2016年 / 17卷 / 19期

关键词：

conjugation; CRISPR-Cas9; gene technology; oligonucleotides; single guide RNA; SOLID-PHASE; HUMAN-CELLS; GENOME; CAS9; CHEMISTRY; ENDONUCLEASE; DELIVERY; SYSTEMS; DNA;

D O I：

10.1002/cbic.201600320

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CRISPR-Cas9 gene editing system requires Cas9 endonuclease and guide RNAs (either the natural dual RNA consisting of crRNA and tracrRNA or a chimeric single guide RNA) that direct site-specific double-stranded DNA cleavage. This communication describes a click ligation approach that uses alkyne-azide cycloaddition to generate a triazole-linked single guide RNA (sgRNA). The conjugated sgRNA shows efficient and comparable genome editing activity to natural dual RNA and unmodified sgRNA constructs.

引用

页码：1809 / 1812

页数：4

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