Bleach-stable, alkaline protease from Bacillus sp.

被引:114
作者
Gupta, R [1 ]
Gupta, K [1 ]
Saxena, RK [1 ]
Khan, S [1 ]
机构
[1] Univ Delhi, Dept Microbiol, New Delhi 110021, India
关键词
bleach-stable; thermotolerant; alkaline protease; Bacillus sp;
D O I
10.1023/A:1005478117918
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A bleach-stable, thermotolerant, alkaline protease for detergent formulation from a newly isolated Bacillus SB5 is reported. Most (85%) activity of the enzyme was retained in the presence of 10% (v/v) H2O2 and 1% SDS (w/v) at 40 degrees C, after 1 h. The enzyme was optimal at pH 10 and 60 degrees C to 70 degrees C. Enzyme activity was enhanced 30 to 80%; in presence of ionic and non-ionic detergents, surfactants and commercial detergents or bleach.
引用
收藏
页码:135 / 138
页数:4
相关论文
共 15 条
[11]   HIGH-ACTIVITY ALKALINE PROTEASE FROM CONIDIOBOLUS-CORONATUS (NCL 86.8.20) - ENZYME-PRODUCTION AND COMPATIBILITY WITH COMMERCIAL DETERGENTS [J].
PHADATARE, SU ;
DESHPANDE, VV ;
SRINIVASAN, MC .
ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (01) :72-76
[12]  
TAKAMI H, 1989, APPL MICROBIOL BIOT, V30, P120
[13]   PRODUCTION AND FURTHER CHARACTERIZATION OF AN ALKALINE ELASTASE PRODUCED BY ALKALOPHILIC BACILLUS STRAIN YA-B [J].
TSAI, YC ;
JUANG, RY ;
LIN, SF ;
CHEN, SW ;
YAMASAKI, M ;
TAMURA, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (12) :3156-3161
[14]  
Tuschiya K., 1992, BIOSCI BIOTECH BIOCH, V56, P246
[15]  
Wolff AM, 1996, ADV EXP MED BIOL, V379, P113