A quantitative liposome microarray to systematically characterize protein-lipid interactions

被引：65

作者：

Saliba, Antoine-Emmanuel ^{[1
]}

Vonkova, Ivana ^{[1
]}

Ceschia, Stefano ^{[1
]}

Findlay, Greg M. ^{[2
]}

Maeda, Kenji ^{[1
]}

Tischer, Christian ^{[3
]}

Deghou, Samy ^{[1
]}

van Noort, Vera ^{[1
]}

Bork, Peer ^{[1
]}

Pawson, Tony ^{[2
]}

Ellenberg, Jan ^{[4
]}

Gavin, Anne-Claude ^{[1
]}

机构：

[1] European Mol Biol Lab, Struct & Computat Biol Unit, Heidelberg, Germany

[2] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[3] European Mol Biol Lab, Adv Light Microscopy Facil, Heidelberg, Germany

[4] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

来源：

NATURE METHODS | 2014年 / 11卷 / 01期

关键词：

BINDING DOMAINS; MEMBRANE; DYNAMICS; BILAYERS;

D O I：

10.1038/nmeth.2734

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

引用

页码：47 / +

页数：6

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