Aberrant histone modification in peripheral blood B cells from patients with systemic sclerosis

被引：55

作者：

Wang, YaoYao ^{[1
]}

Yang, Yan ^{[1
]}

Luo, YangYang ^{[1
]}

Yin, YongXin ^{[1
]}

Wang, Qing ^{[1
]}

Li, YaPing ^{[1
]}

Kanekura, Takuro ^{[3
]}

Wang, JiuCun ^{[4
,5
]}

Liang, GongPing ^{[1
,2
]}

Zhao, Ming ^{[1
,2
]}

Lu, QianJin ^{[1
,2
]}

Xiao, Rong ^{[1
]}

机构：

[1] Cent S Univ, Xiangya Hosp 2, Dept Dermatol, Changsha 410011, Hunan, Peoples R China

[2] Hunan Key Lab Med Epigen, Changsha 410011, Hunan, Peoples R China

[3] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Dermatol, Kagoshima 8908520, Japan

[4] Fudan Univ, Sch Life Sci, Minist Educ MOE, Key Lab Contemporary Anthropol, Shanghai 200433, Peoples R China

[5] Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

来源：

CLINICAL IMMUNOLOGY | 2013年 / 149卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Histone modification; Acetylation; Methylation; B cells; Systemic sclerosis; EPIGENETIC REGULATION; T-CELLS; LYMPHOCYTE; RECEPTOR; AUTOIMMUNITY; ACTIVATION; DISEASE; SKIN; ACCESSIBILITY; PATHOGENESIS;

D O I：

10.1016/j.clim.2013.06.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Objectives: To investigate alterations in histone modifications in B cells and their role in the pathogenesis of systemic sclerosis (SSc). Methods: Global histone H3/H4 acetylation and H3K4/H3K9 methylation in B cells of SSc were tested by EpiQuik (TM) assay kits. Related histone modifier enzymes were measured by RT-PCR and Western blot. Results: Global histone H4 hyperacetylation and global histone H3K9 hypomethylation were observed in SSc B cells compared with controls. Expression of JHDM2A was significantly increased but HDAC2, HDAC7, and, SUV39H2 were significantly down-regulated in SSc B cells relative to controls. Global histone H4 acetylation and the expression of HDAC2 were negatively correlated. Global histone H3K9 methylation and the expression of SUV39H2 protein were positively correlated. Global H4 acetylation was positively correlated with disease activity and expression of HDAC2 protein was negatively correlated with skin thickness. Conclusions: Histone modifications were altered in B cells in SSc correlating with skin thickness and disease activity. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：46 / 54

页数：9

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