Mapping and analysis of phosphorylation sites: a quick guide for cell biologists

被引：173
作者：
Dephoure, Noah [1 ]
Gould, Kathleen L. [2 ]
Gygi, Steven P. [1 ]
Kellogg, Douglas R. [3 ]
机构：
[1] Harvard Univ, Dept Cell Biol, Sch Med, Boston, MA 02115 USA
[2] Vanderbilt Univ, Med Ctr, Dept Cell & Dev Biol, Nashville, TN 37232 USA
[3] Univ Calif Santa Cruz, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA
来源：
MOLECULAR BIOLOGY OF THE CELL | 2013年 / 24卷 / 05期
关键词：
MASS-SPECTROMETRY; SACCHAROMYCES-CEREVISIAE; PROTEIN-PHOSPHORYLATION; WIDE PREDICTION; TANDEM; IDENTIFICATION; PROTEOMICS; STRATEGY; COMPLEX; BINDING;
D O I：
10.1091/mbc.E12-09-0677
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.
引用
收藏
页码：535 / 542
页数：8
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