Mapping and analysis of phosphorylation sites: a quick guide for cell biologists

被引：173

作者：

Dephoure, Noah ^{[1
]}

Gould, Kathleen L. ^{[2
]}

Gygi, Steven P. ^{[1
]}

Kellogg, Douglas R. ^{[3
]}

机构：

[1] Harvard Univ, Dept Cell Biol, Sch Med, Boston, MA 02115 USA

[2] Vanderbilt Univ, Med Ctr, Dept Cell & Dev Biol, Nashville, TN 37232 USA

[3] Univ Calif Santa Cruz, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA

来源：

MOLECULAR BIOLOGY OF THE CELL | 2013年 / 24卷 / 05期

关键词：

MASS-SPECTROMETRY; SACCHAROMYCES-CEREVISIAE; PROTEIN-PHOSPHORYLATION; WIDE PREDICTION; TANDEM; IDENTIFICATION; PROTEOMICS; STRATEGY; COMPLEX; BINDING;

D O I：

10.1091/mbc.E12-09-0677

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.

引用

页码：535 / 542

页数：8

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