Improving the understanding of oligonucleotide-nanoparticle conjugates using DNA-binding fluorophores

被引：0
|
作者：
Guerrini, Luca [1 ]
Barrett, Lee [1 ]
Dougan, Jennifer A. [1 ]
Faulds, Karen [1 ]
Graham, Duncan [1 ]
机构：
[1] Univ Strathclyde, Dept Pure & Appl Chem, Ctr Mol Nanometrol, Glasgow G1 1YL, Lanark, Scotland
来源：
NANOSCALE | 2013年 / 5卷 / 10期
关键词：
METAL-ENHANCED FLUORESCENCE; SYBR-GREEN-I; GOLD NANOPARTICLES; SURFACE COVERAGE; HYBRIDIZATION; MOLECULES; SILVER; PCR;
D O I：
10.1039/c3nr01197f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel fluorescent-based method for characterisation of oligonucleotide-nanoparticle conjugates (ONPCs) is reported. We exploit the ability of the double-stranded DNA-binding dye, SYBR Green I, to develop a powerful analytical tool to investigate the melting properties and hybridisation behavior of ONPCs as well as their corresponding DNA-mediated assemblies.
引用
收藏
页码：4166 / 4170
页数：5
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