A sensitive, homogeneous fluorescence assay for detection of thymine DNA glycosylase activity based on exonuclease-mediated amplification

被引：30

作者：

Chen, Cuihua ^{[1
]}

Zhou, Dianming ^{[1
]}

Tang, Hao ^{[1
]}

Liang, Manfen ^{[1
]}

Jiang, Jianhui ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 52期

关键词：

EXCISION-REPAIR; N-GLYCOSYLASE; MAMMALIAN DNA; DEMETHYLATION; 5-METHYLCYTOSINE; CONVERSION; 5-CARBOXYLCYTOSINE; METHYLATION; DEAMINATION; TET1;

D O I：

10.1039/c3cc41700j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel homogeneous fluorescence assay strategy for highly sensitive detection of thymine DNA glycosylase (TDG) enzyme activity based on the exonuclease-mediated signal amplification reaction was reported.

引用

页码：5874 / 5876

页数：3

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