Screening Procedures for Chloramphenicol Residue Determination in Serum, Milk, and Feedstuff Using Immunochromatographic Assay

被引：7

作者：

Hsieh, K. H. ^{[1
]}

Lin, C. P. ^{[1
]}

Cheng, C. C. ^{[1
]}

Lai, C. P. ^{[1
]}

Sheu, S. Y. ^{[2
,3
,4
]}

Yao, C. H. ^{[2
,5
,6
]}

Lei, Y. C. ^{[1
,7
]}

Chang, T. H. ^{[1
]}

Kuo, T. F. ^{[7
]}

机构：

[1] Taiwan Advance Biopharmaceut Inc, New Taipei City, Taiwan

[2] China Med Univ, Sch Chinese Med, Taichung, Taiwan

[3] Chung Shan Med Univ, Sch Med, Taichung, Taiwan

[4] Chung Shan Med Univ Hosp, Dept Integrated Chinese & Western Med, Taichung, Taiwan

[5] China Med Univ, Dept Biomed Imaging & Radiol Sci, Taichung, Taiwan

[6] Asia Univ, Dept Biomed Informat, Taichung, Taiwan

[7] Natl Taiwan Univ, Dept Vet Med, Taipei 106, Taiwan

来源：

FOOD BIOTECHNOLOGY | 2013年 / 27卷 / 04期

关键词：

colloidal gold-based immunochromatographic assay (CGIA); lateral flow immunoassay; chloramphenicol; residue; food safety; LINKED-IMMUNOSORBENT-ASSAY; MASS-SPECTROMETRY; FLORFENICOL AMINE; THIAMPHENICOL;

D O I：

10.1080/08905436.2013.838784

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The technology developed from this study is based on an immunochromatographic procedure that utilizes antigen-antibody properties using membrane carriers with immobilized immunoreactants and provides rapid and out-of-laboratory techniques. An evaluation of a colloidal gold-based immunochromatographic assay for chloramphenicol (CAP) residue detection in serum, milk and feed is described. Polyclonal antibodies against CAP were produced using CAP-IPA-MAA-ABA-bovine serum albumin (CAP-ed-BSA) conjugate as the immunogen, which exhibited no cross-reactivities with applied competitors in the studied concentration range. The test strip assay could be completed within 7 min, with a visual detection limit of 0.3 g kg(-1) CAP in serum and milk and 0.5 g kg(-1) CAP in feed. The established strip assay performed similarly as a commercial ELISA assay: the CAP test strip revealed a sensitivity of 90% (89-92%) and a specificity of 94% (91-98%), which showed appreciable accuracy and precision. The described strip is rapid, simple and cost-effective as well as sensitive and specific enough for reliable and accurate on-site screening.

引用

页码：328 / 341

页数：14

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