Analysis of lactic acid bacteria communities and their seasonal variations in a spontaneously fermented dairy product (Malian fene) by applying a cultivation/genotype-based binary model

被引：33
作者：
Wullschleger, Stephan [1 ]
Lacroix, Christophe [1 ]
Bonfoh, Bassirou [2 ]
Sissoko-Thiam, Aminata [3 ]
Hugenschmidt, Selina [1 ]
Romanens, Edwina [1 ]
Baumgartner, Sarah [1 ]
Traore, Idrissa [3 ]
Yaffee, Marcus [1 ]
Jans, Christoph [1 ]
Meile, Leo [1 ]
机构：
[1] Swiss Fed Inst Technol, Inst Food Nutr & Hlth, Lab Food Biotechnol, CH-8092 Zurich, Switzerland
[2] CSRS, Abidjan, Cote Ivoire
[3] LCV, Bamako, Mali
来源：
INTERNATIONAL DAIRY JOURNAL | 2013年 / 29卷 / 01期
关键词：
16S RIBOSOMAL-RNA; FIELD GEL-ELECTROPHORESIS; PCR-BASED METHOD; RAPID IDENTIFICATION; STREPTOCOCCUS-THERMOPHILUS; ANTIBIOTIC-RESISTANCE; EQUINUS COMPLEX; MILK-PRODUCTS; GENE-TRANSFER; BAMAKO MALI;
D O I：
10.1016/j.idairyj.2012.08.001
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Seasonal fluctuations of lactic acid bacteria (LAB) communities were analysed in spontaneous fermentation processes of the Malian sour milk fene through the design of a novel flow-chart protocol for rapid typing of putative LAB. The protocol is based on cultivation on semi-selective complex agar media, followed by a binary scheme for genus-and species-specific genotypic identification. Presumptive LAB (n = 1583) were isolated and typed from 98 samples. Fene samples displayed 10(8) cfu mL(-1) in all seasons. Seasonal fluctuations indicated a higher prevalence of Streptococcus spp. and Enterococcus spp. in the cold and hot season, respectively. Analysis of enterococci (n = 596) and streptococci (n = 454) revealed the predominance of Streptococcus infantarius subsp. infantarius over Streptococcus thermophilus. The binary culture-based protocol is a rapid tool to follow LAB species diversity and seasonal variations in any complex dairy microbial ecosystem including the cultivation of strains for subsequent characterisation. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：28 / 35
页数：8
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