Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnoideae and Bromelioideae

被引：8

作者：

de Miranda, Fabio Demolinari ^{[1
]}

Passos Lima Gontijo, Andreia Barcelos ^{[2
]}

Santiliano, Fabiano Costa ^{[1
]}

Favoreto, Fernanda Campanharo ^{[3
]}

Bastos Soares, Tais Cristina ^{[4
]}

机构：

[1] UFES, Ctr Ciencias Agr, Dept Biol, BR-29500000 Alegre, ES, Brazil

[2] UFES, Ctr Univ No Espirito Santo, Dept Ciencias Agr & Biol, BR-29932540 Sao Mateus, ES, Brazil

[3] Univ Fed Juiz de Fora, Inst Ciencias Biol, Programa Posgrad Ecol, BR-36036900 Juiz de Fora, MG, Brazil

[4] UFES, Ctr Ciencias Agr, Dept Farm & Nutr, BR-29500000 Alegre, ES, Brazil

来源：

BIOTA NEOTROPICA | 2012年 / 12卷 / 03期

关键词：

cross-amplification; SSR; genetic diversity; bromeliads; conservation; SIMPLE SEQUENCE REPEATS; CROSS-AMPLIFICATION; GENETIC-ANALYSIS; SSR-MARKERS; GENUS; POLYMORPHISM; DIVERSITY; BARLEY; LOCI;

D O I：

10.1590/S1676-06032012000300032

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. H. values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult & Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for R albiflos and? geysksii effectively amplified the DNA samples of Pitcairnia flammea,Aechmea nudicaulis(L.) Griseb., Aechmea ramosa, Billbergia horrida and Billbergia euphemiae, validating the transferability of these markers to species of the Pitcairnioideae and Bromelioideae subfamilies.

引用

页码：319 / 323

页数：5

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