Cloning of porcine IGF1 receptor cDNA and detection of sequence polymorphisms using RT-PCR

被引：7

作者：

Harumi, T ^{[1
]}

Maruyama, K

Kagami, H

Sano, A

Matsubara, Y

Tagami, T

Naito, M

机构：

[1] Natl Inst Agrobiol Sci, Anim Genet Engn Lab, Tsukuba, Ibaraki 3050901, Japan

[2] Meiji Univ, Fac Agr, Dept Life Sci, Tama Ku, Tokyo 101, Japan

[3] Shinshu Univ, Fac Agr, Nagano, Japan

来源：

ANIMAL GENETICS | 2001年 / 32卷 / 06期

关键词：

insulin-like growth factor 1; polymorphism; porcine; receptor; type 1 insulin-like growth factor receptor;

D O I：

10.1046/j.1365-2052.2001.00793.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A cDNA encoding the porcine type 1 insulin-like growth factor receptor (IGF1R) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). The sequence of a 4.2-kb product was determined and had an open reading frame, encoding 1367 amino acids with 98.1 and 95.2% sequence similarity to the human and rat IGF1R, respectively. In the comparison of RT-PCR derived IGF1R sequences from 12 unrelated pigs, 12 silent sequence variants were found.

引用

页码：386 / 389

页数：4

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