The E-coli α-hemolysin secretion system and its use in vaccine development

被引：124

作者：

Gentschev, I ^{[1
]}

Dietrich, G

Goebel, W

机构：

[1] Univ Wurzburg, Dept Microbiol, D-97074 Wurzburg, Germany

[2] Berna Biotech Ltd, Bacterial Vaccine Res, CH-3018 Bern, Switzerland

来源：

TRENDS IN MICROBIOLOGY | 2002年 / 10卷 / 01期

关键词：

D O I：

10.1016/S0966-842X(01)02259-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many Gram-negative bacteria use a type I secretion system to translocate proteins, including pore-forming toxins, proteases, lipases and S-layer proteins, across both the inner and outer membranes into the extracellular surroundings. The Escherichia colialpha-hemolysin (HIyA) secretion system is the prototypical and best characterized type I secretion system. The structure and function of the components of the HIyA secretion apparatus, HIyB, HIyD and TolC, have been studied in great detail. The functional characteristics of this secretion system enable it to be used in a variety of different applications, including the presentation of heterologous antigens in live-attenuated bacterial vaccines. Such vaccines can be an effective delivery system for heterologous antigens, and the use of a type 1 secretion system allows the antigens to be actively exported from the cytoplasm of the bacterial carrier rather than only becoming accessible to the host immune system after bacterial disintegration.

引用

页码：39 / 45

页数：7

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