MALDI-TOF MS Andromas strategy for the routine identification of bacteria, mycobacteria, yeasts, Aspergillus spp. and positive blood cultures

被引:100
|
作者
Bille, E. [1 ,2 ]
Dauphin, B. [3 ]
Leto, J. [1 ]
Bougnoux, M. -E. [1 ,2 ]
Beretti, J. -L. [1 ]
Lotz, A. [2 ]
Suarez, S. [1 ]
Meyer, J. [2 ]
Join-Lambert, O. [1 ,2 ]
Descamps, P. [1 ,2 ]
Grall, N. [1 ]
Mory, F. [4 ]
Dubreuil, L. [5 ]
Berche, P. [1 ,2 ]
Nassif, X. [1 ,2 ]
Ferroni, A. [1 ]
机构
[1] Hop Necker Enfants Malad, AP HP, Microbiol Lab, F-75015 Paris, France
[2] Univ Paris 05, Fac Med, Paris, France
[3] Andromas SAS, Paris, France
[4] Fac Med Vandoeuvre Nancy, Lab Bacteriol Virol, Vandoeuvre Les Nancy, France
[5] Fac Pharm Lille, Microbiol Clin, Lille, France
关键词
Aspergillus ssp; bacteria; blood cultures; identification; MALDI-TOF; mass spectrometry; medical laboratory; mycobacteria; yeast; LASER-DESORPTION IONIZATION; FLIGHT MASS-SPECTROMETRY; SEQUENCE-BASED IDENTIFICATION; RAPID IDENTIFICATION; MICROBIOLOGY LABORATORIES; SPECIES IDENTIFICATION; TIME; STREPTOCOCCUS; STAPHYLOCOCCI; BOTTLES;
D O I
10.1111/j.1469-0691.2011.03688.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Clin Microbiol Infect 2012; 18: 11171125 Abstract All organisms usually isolated in our laboratory are now routinely identified by matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS) using the Andromas software. The aim of this study was to describe the use of this strategy in a routine clinical microbiology laboratory. The microorganisms identified included bacteria, mycobacteria, yeasts and Aspergillus spp. isolated on solid media or extracted directly from blood cultures. MALDI-TOF MS was performed on 2665 bacteria isolated on solid media, corresponding to all bacteria isolated during this period except Escherichia coli grown on chromogenic media. All acquisitions were performed without extraction. After a single acquisition, 93.1% of bacteria grown on solid media were correctly identified. When the first acquisition was not contributory, a second acquisition was performed either the same day or the next day. After two acquisitions, the rate of bacteria identified increased to 99.2%. The failures reported on 21 strains were due to an unknown profile attributed to new species (9) or an insufficient quality of the spectrum (12). MALDI-TOF MS has been applied to 162 positive blood cultures. The identification rate was 91.4%. All mycobacteria isolated during this period (22) were correctly identified by MALDI-TOF MS without any extraction. For 96.3% and 92.2% of yeasts and Aspergillus spp., respectively, the identification was obtained with a single acquisition. After a second acquisition, the overall identification rate was 98.8% for yeasts (160/162) and 98.4% (63/64) for Aspergillus spp. In conclusion, the MALDI-TOF MS strategy used in this work allows a rapid and efficient identification of all microorganisms isolated routinely.
引用
收藏
页码:1117 / 1125
页数:9
相关论文
共 50 条
  • [31] "Inactivated mycobacteria bead preparation" method for identification of mycobacteria by MALDI-TOF MS
    Timke, M.
    Kostrzewa, M.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 301 : 1 - 1
  • [32] Identification of Candida yeasts: Conventional methods and MALDI-TOF MS
    Maldonado, Ivana
    Cataldi, Silvana
    Garbasz, Claudia
    Relloso, Silvia
    Striebeck, Pablo
    Guelfand, Liliana
    Lopez Moral, Laura
    REVISTA IBEROAMERICANA DE MICOLOGIA, 2018, 35 (03): : 151 - 154
  • [33] Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry
    Ulrich, Sebastian
    Biermaier, Barbara
    Bader, Oliver
    Wolf, Georg
    Straubinger, Reinhard K.
    Didier, Andrea
    Sperner, Brigitte
    Schwaiger, Karin
    Gareis, Manfred
    Gottschalk, Christoph
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 408 (27) : 7565 - 7581
  • [34] Investigating the critical steps in the identification of yeasts from blood cultures using MALDI-TOF
    Barker, K. R.
    Gorton, R. L.
    Stone, N. R. H.
    Rattenbury, S.
    Kibbler, C. C.
    MYCOSES, 2011, 54 : 75 - 76
  • [35] Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry
    Sebastian Ulrich
    Barbara Biermaier
    Oliver Bader
    Georg Wolf
    Reinhard K. Straubinger
    Andrea Didier
    Brigitte Sperner
    Karin Schwaiger
    Manfred Gareis
    Christoph Gottschalk
    Analytical and Bioanalytical Chemistry, 2016, 408 : 7565 - 7581
  • [36] The identification of anaerobic bacteria using MALDI-TOF MS
    Veloo, A. C. M.
    Welling, G. W.
    Degener, J. E.
    ANAEROBE, 2011, 17 (04) : 211 - 212
  • [37] Utility of MALDI-TOF MS for the identification of anaerobic bacteria
    Zarate, Mariela S.
    Romano, Vanesa
    Nievas, Jimena
    Smayevsky, Jorgelina
    REVISTA ARGENTINA DE MICROBIOLOGIA, 2014, 46 (02): : 98 - 102
  • [38] Evaluation of three sample preparation methods for the direct identification of bacteria in positive blood cultures by MALDI-TOF
    Tanner H.
    Evans J.T.
    Gossain S.
    Hussain A.
    BMC Research Notes, 10 (1) : 1 - 8
  • [39] Implementation of short incubation MALDI-TOF MS identification from positive blood cultures in routine diagnostics and effects on empiric antimicrobial therapy
    Robin Köck
    Jörg Wüllenweber
    Dagmar Horn
    Christian Lanckohr
    Karsten Becker
    Evgeny A. Idelevich
    Antimicrobial Resistance & Infection Control, 6
  • [40] Implementation of short incubation MALDI-TOF MS identification from positive blood cultures in routine diagnostics and effects on empiric antimicrobial therapy
    Koeck, Robin
    Wuellenweber, Joerg
    Horn, Dagmar
    Lanckohr, Christian
    Becker, Karsten
    Idelevich, Evgeny A.
    ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL, 2017, 6